FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Grip84, t-Grip84
FlyBase ID
FBig0000123348
Interaction Network
Interactions Browser links
Grip84 network
t-Grip84 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0260790-5.Y2H
Reference
Alzyoud et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Grip84 

Grip84

bait

fused to GAL4 DNA-binding domain

Grip84 
t-Grip84 

t-Grip84

prey

fused to GAL4 activation domain

t-Grip84 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Grip84 
interaction domain domain
sufficient binding region
aa 1-833
t-Grip84 
C-terminal domain
sufficient binding region
aa 400-728

coordinates relative to t-Grip84-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Alzyoud et al., 2024)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Alzyoud et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)