Flies were maintained at 25oC and a 0-3 hour embryo collection was collected.

The mock immunoprecipitation used an unrelated synthetic FLAG-tagged Fab antibody fragment from the same library as the stau-specific Fab fragment used. Bound protein-RNA was eluted using FLAG peptide. RNA in coimmunoprecipitation was purified using Trizol reagent. Double-stranded cDNA was prepared from isolated RNA using oligo-dT primers and labelled for subsequent hybridization.

Labelled cDNA libraries were characterized by hybridization to custom-designed NimbleGen array (GPL10539).