Omelyanchuk, L.V., Dubovski, I.M., Glupov, V.V. (2001). A regulatory gene for phenoloxidase activity in Drosophila melanogaster.  Genetika, Moscow 37(8): 1063--1067.

FBrf0167665

Research paper

A recessive lethal mutation l(2)hemo causing the occurrence of melanotic tumors in homozygous Drosophila larvae was found. The study of phenoloxidase (PO) activity revealed that the number of hemocytes with PO activity in homozygous larvae was significantly reduced (0.4 +/- 0.24%), compared to wild-type larvae (6.3 +/- 0.5%). On injury followed by injection with bacterial cells, the formation of melanotic thrombus did not occurred and hemocytes with PO activity were not recorded in homozygotes of line P103. Suppression of the activity of PO isozymes A1 and A3 was detected by means of electrophoretic analysis of homozygotes. According to gene mapping data, the localization of this mutation did not match any structural gene for known PO forms and is therefore related to a regulatory gene controlling the activity of the immune system of Drosophila.