Subject: Re: Chanana et al., 2007, Mech. Dev. 124(6): 463--475 FBrf0201519 Dear Dr. Stefancsik, I needed some time to go through the original data, as the mutant was generated about five years ago. According to the original notes the deficiency used wasDf(2R)pk78k, Tp(2;2)CA30, sp1/CyO which was lethal in trans of the P-element and the jump out lines generated. I can only presume that the transposition might have caused a defect in the region of the tsp gene. We did most of the analysis using the jump out alleles to analyze the phenotypes and the rescue experiments. However to exclude secondary hits on the chromosome, we wanted to include the phenotypic analysis over a deficiency as well. As the mutants alleles and the P were lethal over the this deficiency plus transposition line I was happy to have an additional allele and did not analyzed the basis of this into further detail. I am really sorry that we referred to the wrong deficiency in the manuscript. Yours sincerely Gerd Vorbrüggen On Jul 3, 2009, at 4:03 PM, Raymund Stefancsik wrote: >Dear Dr. Vorbrüggen, > >I am writing regarding your paper that I am curating for FlyBase: > >Chanana, B., Graf, R., Koledachkina, T., Pflanz, R., Vorbrüggen, G. (2007). >αPS2 integrin-mediated muscle attachment in Drosophila requires the ECM protein >Thrombospondin. Mech. Dev. 124(6): 463--475. FBrf0201519 > >I am wondering if you could help me identify the deficiency that you call >Df(2)pk78k and Df(2L)pk78k in your paper. You mention in the "Materials and >methods" that you got your deficiencies including Df(2)pk78k from Bloomington. >However, when I search for it, the closest match I get from the Bloomington >collection is Df(2R)pk78k (FBab0002195). > >As Thrombospondin (FBgn0031850) is on 2L, Df(2R)pk78k (FBab0002195) does not >uncover Tsp according to the data we have in FlyBase. However, the in situ >experiments on page 468 appear to suggest that either Df(2L)pk78k does uncover >Tsp or it is a dominant suppressor of Tsp expression. > >I would greatly appreciate if you could clarify the identity of Df(2L)pk78k, so >we can incorporate the data from your paper as accurately as possible. > >Sincerely, > >************************** >Raymund Stefancsik Ph. D. >FlyBase Curator >************************** >
