FB2025_01 , released February 20, 2025
Sequence Feature: Dmel\A-I_edit_000127
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General Information
Symbol
Dmel\A-I_edit_000127
Species
D. melanogaster
Feature type
FlyBase ID
FBsf0000383491
Genomic Location
Chromosome (arm)
X
Sequence location

X:16,505,550..16,505,550 [-]

Genomic Maps
Sequence Data
Length
Comments

Site of A-to-I editing in 3'UTR: para-RAR

Site of A-to-I editing in CDS: Lys 383 to Arg codon substitution in para-RAS.

Site of A-to-I editing in CDS: Lys 383 to Arg codon substitution in para-RAT.

Site of A-to-I editing in CDS: Lys 383 to Arg codon substitution in para-RAU.

Site of A-to-I editing in CDS: Lys 383 to Arg codon substitution in para-RAV.

Site of A-to-I editing in CDS: Lys 425 to Arg codon substitution in para-RBH.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAD.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAF.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAG.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAH.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAI.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAJ.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAK.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAL.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAM.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAW.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAX.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAY.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RAZ.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RBA.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RBB.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RBC.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RBD.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RBE.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RD.

Site of A-to-I editing in CDS: Lys 426 to Arg codon substitution in para-RH.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RA.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RAA.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RAB.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RAC.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RAE.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RAN.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RAO.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RAP.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RAQ.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RB.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RBF.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RBG.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RC.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RE.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RF.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RG.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RI.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RJ.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RK.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RL.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RM.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RN.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RO.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RP.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RQ.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RR.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RS.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RT.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RU.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RV.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RW.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RX.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RY.

Site of A-to-I editing in CDS: Lys 437 to Arg codon substitution in para-RZ.

Associated Information
Experimental Data
RNA Editing Sites
Dataset: mE_A-to-I_RNA_Editing_Sites
Expression stage(s)
Read Count
Editing Frequency
embryonic stage 1 -- 4

Comment: 0-2 hr AEL

0
0.00
embryonic stage 4 -- 9

Comment: 2-4 hr AEL

0
0.00
embryonic stage 9 -- 11

Comment: 4-6 hr AEL

0
0.00
embryonic stage 11 -- 12

Comment: 6-8 hr AEL

0
0.00
embryonic stage 12 -- 13

Comment: 8-10 hr AEL

0
0.00
embryonic stage 13 -- 15

Comment: 10-12 hr AEL

0
0.00
embryonic stage 15 -- 16

Comment: 12-14 hr AEL

1
0.00
embryonic stage 16

Comment: 14-16 hr AEL

2
0.00
embryonic stage 17(i)

Comment: 16-18 hr AEL

13
0.00
embryonic stage 17(ii)

Comment: 18-20 hr AEL

30
0.20
embryonic stage 17(iii)

Comment: 20-22 hr AEL

14
0.00
embryonic stage 17(iv)

Comment: 22-24 hr AEL

23
0.35
first instar larval stage

Comment: 42-44hr AEL

21
0.00
second instar larval stage

Comment: 66-70h AEL

10
0.70
2
0.00
third instar - uncleared gut stage

Comment: indicated as puff stage 1-2

0
0.00
third instar - partially cleared gut stage

Comment: indicated as puff stage 3-6

0
0.00
third instar - cleared gut stage

Comment: indicated as puff stage 7-9

2
0.00
pupal stage P5

Comment: aged 12 hrs after collection as white prepupae

1
0.00
pupal stage P6

Comment: aged 24 hrs after collection as white prepupae

2
0.00
pharate adult stage P8

Comment: aged 2 days after collection as white prepupae

8
0.00
pharate adult stage P9 -- P10

Comment: aged 3 days after collection as white prepupae

16
0.69
pharate adult stage P15

Comment: aged 4 days after collection as white prepupae

3
0.00
day 1 of adulthood | male

Comment: aged 1 day after eclosion

9
0.56
day 1 of adulthood | female

Comment: aged 1 day after eclosion

4
0.00
day 5 of adulthood | male

Comment: aged 5 days after eclosion

10
0.90
day 5 of adulthood | female

Comment: aged 5 days after eclosion

1
0.00
day 30 of adulthood | male

Comment: aged 30 days after eclosion

8
0.88
day 30 of adulthood | female

Comment: aged 30 days after eclosion

0
0.00

Total
180
Dataset: Rosbash_Adult_Head_A-to-I_Editing
Expression stage(s)
Read Count
Editing Frequency
day 3 of adulthood -- day 4 of adulthood

Comment: D. melanogaster, yw, adult head, ZT10, source for nuclear RNA.

34
0.66

Total
34
Collection Information
Collection: mE_A-to-I_RNA_Editing_Sites
Title
A-to-I RNA editing site identification from RNA-Seq assays of D. melanogaster, iso-1 strain, 30 developmental stages.
Source and Progenitors
Species of derivation
D. melanogaster
Strain of derivation
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: aged 12 hrs after collection as white prepupae

organism

Comment: aged 24 hrs after collection as white prepupae

organism

Comment: aged 2 days after collection as white prepupae

organism

Comment: aged 3 days after collection as white prepupae

organism

Comment: aged 4 days after collection as white prepupae

organism

Comment: aged 1 day after eclosion

organism

Comment: aged 1 day after eclosion

organism

Comment: aged 5 days after eclosion

organism

Comment: aged 5 days after eclosion

organism

Comment: aged 30 days after eclosion

organism

Comment: aged 30 days after eclosion

Vector of progenitor construct
Description

RNA A-to-I editing sites as determined from short sequencing reads of cDNA prepared from 30 different developmental stages. The frequency of editing at each site throughout development is analyzed and the effects of editing on the amino acids encoded are predicted.

Additional data
Sample preparation
Collection preparation
Mode of assay
Data analysis

To identify potential sites of RNA editing, short poly(A)+ RNA-Seq reads that mapped to annotated transcripts were compared to the reference genome to find instances in which a 'G' aligned to an 'A' (for plus strand transcripts) or a 'C' aligned to a 'T' (for minus strand). The first or last six bases of reads were not examined because they were easily mismapped to introns rather than across splice junctions. Only positions with at least five reads showing a substitution from any single developmental stage were considered further. A dataset of potential editing sites with the number of edited reads and the number of non-edited reads for each sample was created. To prevent a polymorphism in the sequenced strain from being reported as editing, at least 100 reads having an exact match to the genome were necessary for further consideration of a potential editing site. Only those sites with at least 5% of reads in at least two independent adult samples showing evidence of editing are reported.

Cell lines
Observed in
Not observed in
Collection: Rosbash_Adult_Head_A-to-I_Editing
Title
RNA-Seq identification of cotranscriptional RNA editing sites in nascent transcripts of adult head.
Source and Progenitors
Species of derivation
D. melanogaster
Strain of derivation
Stage
Tissue/Position (including subcellular localization)
Reference
adult head

Comment: D. melanogaster, yw, adult head, ZT14, source for RNA.

adult head
nucleus

Comment: D. melanogaster, yw, adult head, ZT6, source for nuclear RNA.

Vector of progenitor construct
Description
Additional data
Sample preparation

Flies were entrained under 12hr:12hr light:dark cycles for 3-4 days.

Collection preparation
Mode of assay
Data analysis
Cell lines
Observed in
Not observed in
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
A-I_edit_000127
chrX:16399583
Secondary FlyBase IDs
    References (3)