FB2025_01 , released February 20, 2025
Sequence Feature: Dmel\sgRNA-GS05334
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General Information
Symbol
Dmel\sgRNA-GS05334
Species
D. melanogaster
Feature type
sgRNA
FlyBase ID
FBsf0000766790
Collection
Associated gene
Genomic Location
Chromosome (arm)
3R
Sequence location

3R:28,820,175..28,820,197 [-]

Genomic Maps
Sequence Data
Length
23
Comments

Sequence

Sequence Downloader
GAAATCGATGTGCTATCGATAGG
Associated Information
Gene(s) (targeted or local)
Transcripts(s)
    Polypeptide(s)
      Experimental Data
      Collection Information
      Collection: TRiP-OE-flySAM.dCas9
      Symbol
      TRiP-OE-flySAM.dCas9
      Title
      A set of transgenic sgRNA + dCas9 constructs for targeted gene overexpression by flySAM.
      Source and Progenitors
      Species of derivation
      D. melanogaster
      Strain of derivation
      Vector of progenitor construct
      Description

      A set of transgenic constructs that each consist of single sgRNA that contains MS2 loops. The tagged sgRNA forms one component of the three-component flySAM (synergistic activation mediator) system, which is designed to produce effective transcriptional activation of the gene targeted by the sgRNA. In this collection, each construct also contains the other two components of the flySAM system (nuclease-dead Cas9 fused to a transcriptional activation domain plus an MCP-tagged transcriptional activator), and so target gene expression can be activated by crossing to any GAL4 line.

      (Transgenic RNAi Project members, 2017-)
      Additional data
      Sample preparation
      Collection preparation
      Mode of assay
      Data analysis
      Cell lines
      Observed in
      Not observed in
      Comments
      Stocks (1)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sgRNA-GS05334
      Secondary FlyBase IDs
        References (1)