FB2025_01 , released February 20, 2025
Sequence Feature: Dmel\sgRNA-NIG-Fly.achi
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General Information
Symbol
Dmel\sgRNA-NIG-Fly.achi
Species
D. melanogaster
Feature type
sgRNA
FlyBase ID
FBsf0000918788
Collection
Associated gene
Genomic Location
Chromosome (arm)
2R
Sequence location

2R:12,513,847..12,513,866 [+]

2R:12,510,058..12,510,077 [+]

Genomic Maps

Multiple sequence regions exist. Please click on a sequence location for a JBrowse view of the region.

2R:12,513,847..12,513,866 [+]

2R:12,510,058..12,510,077 [+]

Sequence Data
Length
20
Comments

Sequence

Sequence Downloader
ACGCTTTAAAATCTTTACAG
Associated Information
Gene(s) (targeted or local)
Allele(s)
Transcripts(s)
    Polypeptide(s)
      Experimental Data
      Collection Information
      Collection: NIG-Fly-sgRNA
      Symbol
      NIG-Fly-sgRNA
      Title
      A set of transgenic constructs for sgRNA-targeted gene mutation.
      Source and Progenitors
      Species of derivation
      D. melanogaster
      Strain of derivation
      Vector of progenitor construct
      Description

      A set of transgenic constructs that each consist of a single guide RNA (sgRNA) targeting a gene of interest for mutation. The sgRNA is expressed under the control of a U6 (ubiquitous) promoter. When crossed to a stock that contains a source of Cas9, cleavage and mutagenesis of the targeted gene is induced, resulting in a small deletion or insertion mutation.

      (National Institute of Genetics Fly Stocks, 2022-)
      Additional data
      Sample preparation
      Collection preparation
      Mode of assay
      Data analysis
      Cell lines
      Observed in
      Not observed in
      Comments
      Stocks (1)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      sgRNA-NIG-Fly.achi
      Secondary FlyBase IDs
        References (1)