Dmel\P{bluetail}Trl^13C Insertion

General Information
Symbol	Dmel\P{bluetail}Trl^13C	Species	D. melanogaster
Name		FlyBase ID	FBti0013362
Feature type	transposable_element_insertion_site
Description
Inserted element	P{bluetail}	Expression data
Affected gene(s)	Trl	Viability / fertility
Causes allele(s)	Trl^13C	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3L ( 70F4 )	Sequence location	3L:14,746,987..14,750,181
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:14,746,987..14,750,181 (Farkas et al., 1994) 3L:14,748,430..14,748,430 [-] (Katokhin et al., 2001) 3L:14,748,430..14,748,430 [-] (Katokhin et al., 2001)
Orientation	- (Farkas et al., 1994, Katokhin et al., 2001, Katokhin et al., 2001)
Cytological location (computed by FlyBase)	70F4 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location	The insertion site was mapped to an EcoRI-BamHI restriction fragment. The orientation of the insertion was not reported. (Farkas et al., 1994)
Sequence Data
Flanking sequence
Inserted Element
Construct	P{bluetail}
Location-dependent role
Size
Associated alleles	Ecol\lacZ^blt ry^+t*
Molecular map
Affected Gene(s)
Insertion may affect gene	Trl (Farkas et al., 1994, Farkas et al., 1994)
Alleles and Phenotypes
Causes alleles	Trl^13C (Farkas et al., 1994, Brumby et al., 2002, Greenberg et al., 2004, Bhat et al., 1996)

Lethality References lethal (Greenberg and Schedl, 2001) lethal \| male (Greenberg et al., 2004) lethal \| maternal effect \| recessive (Bhat et al., 1996) lethal \| recessive (Liaw et al., 1995, Katokhin et al., 2001, Granok et al., 2001) partially lethal - majority die (Greenberg and Schedl, 2001) partially lethal - majority die \| embryonic stage \| maternal effect (Greenberg and Schedl, 2001) viable \| female \| reduced (Greenberg et al., 2004) viable \| recessive \| reduced (Greenberg et al., 2004)
Sterility References female sterile (Farkas et al., 1994) semi-fertile (Greenberg and Schedl, 2001)
Phenotype Manifest In
	abdominal segment 5 \| ectopic (Greenberg and Schedl, 2001) abdominal segment 6 (Shimojima et al., 2003, Greenberg and Schedl, 2001) egg chamber (Trunova et al., 2001) eye (Farkas et al., 1994) imaginal disc (Bejarano and Busturia, 2004) microchaeta & abdominal segment 6 \| ectopic (Shimojima et al., 2003) mitosis & nuclear chromosome (Trunova et al., 2001) sex comb (Shimojima et al., 2003)
Detailed Description
Statement Reference Homozygous Trl^13C larvae survive and their imaginal discs are normal. Trl^R85/Trl^13C larvae do develop till third instar, though their imaginal discs are very small. (Bejarano and Busturia, 2004) Homozygotes have a slightly reduced viability, equal for males and females. Trl^13C/Trl⁶² and Trl^13C/Trl^2.3 animals however, females have a similar viability to homozygotes, males show a significantly reduced viability. (Greenberg et al., 2004) lolal^k02512 ; Trl^13C heterozygous flies are viable and do not show any visible phenotype. Approximately 4% of lolal^k02512/+; Trl^13C/Trl^13C flies hatch. Adding at least one copy of the Hsp83^GAGA519 or Hsp83^GAGA581 transgenes allows the recovery of a much higher frequency of homozygous Trl^13C escapers that are also heterozygous for lolal^k02512 (up to approximately 80% when four copies of the transgene are added). lolal^k02512/+; Trl^13C/Ubx¹³⁰ flies display a higher frequency (18%) of strong haltere-to-wing transformations compared to Trl^13C/Ubx¹³⁰ siblings (6.3%). This increased transformation is often accompanied by a notal transformation. (Faucheux et al., 2003) Approximately 12% of Trl^13C homozygous flies hatch. (Faucheux et al., 2003) Adding at least one copy of the Trl[Hsp83.GAGA-519] or Hsp83[GAGA581] transgenes allows the recovery of a much higher frequency of homozygous Trl[13C] escapers that are also heterozygous for lolal[k02512] (up to approximately 80% when four copies of the transgene are added). (Faucheux et al., 2003) Trl^13C/+, lolal^k02512/+ animals show no enhancement or suppression of the lolal^k02512 phenotype. (Mishra et al., 2003) 56% of Ubx¹³⁰, Trl^13C double heterozygotes have halteres that quite heterogeneous in size and larger than Ubx¹³⁰ alone. 78% of Ubx¹³⁰, Df(3L)Aprt-21, Trl^13C triple heterozygotes have halteres that quite heterogeneous in size and larger than Ubx¹³⁰ alone, also exhibiting a stronger phenotype than any two of the alleles used. The haltere becomes a wing -like structure with wing veins and hairs on the wing margin. Trl^13C Df(3L)Aprt-21 double heterozygotes have an average of about 7 sex combs. 67% of animals have bristles on segment abdominal segment 6. (Shimojima et al., 2003) Heterozygotes have an average of about 10 sex combs, compared to about 11 in wild-type. 11% of animals have bristles on segment abdominal segment 6 (wild-type have none). (Shimojima et al., 2003) Mutant strongly suppresses the CycE^JP rough eye phenotype. (Brumby et al., 2002) Expression of Trl[Hsp83.GAGA-519] partially rescues the lethality seen in Trl[13C]/Trl[R67] mutants, with a survival rate of between 10 and 20% depending on the insertion line. Expression of Trl[Hsp83.GAGA-581] partially rescues the lethality seen in Trl[13C]/Trl[R67] mutants, with a survival rate of between 25 and 60% depending on the insertion line. Expression of a single copy of Trl[Hsp83.GAGA-519] partially rescues the abdominal segment transformation seen in Trl[13C] mutant males. Expression of a single copy of Trl[Hsp83.GAGA-581] does not rescue the abdominal segment transformation seen in Trl[13C] mutant males. Expression of a single copy of Trl[Hsp83.GAGA-519] partially rescues the reduction in egg production seen in Trl[13C] mutant females. Expression of a single copy of Trl[Hsp83.GAGA-581] does not rescue the reduction in egg production seen in Trl[13C] mutant females. Expression of Trl[Hsp83.GAGA-519] partially rescues the lethality seen in embryos produced by homozygous Trl[13C] mutant mothers. Expression of Trl[Hsp83.GAGA-581] does not rescue the lethality seen in embryos produced by homozygous Trl[13C] mutant mothers. Expression of a single copy of Trl[Hsp83.GAGA.T:Ecol\lacZ] rescues the zygotic lethality in Trl[13C]/Trl[R67] mutants. However the A6 to A5 abdominal transformation or the maternal-effect lethality are rescued. (Greenberg and Schedl, 2001) Homozygous Trl[13C] mutant flies have reduced viability and this lethality is enhanced in Trl[13C]/Trl[R67] heterozygotes, with only a few percent of the mutant flies surviving to the adult stage. Approximately 80% of homozygous Trl[13C] mutant males show a transformation of the sixth abdominal segment towards the fifth. Homozygous Trl[13C] mutant females produce a reduced number of eggs compared to wild type. Fewer than 20% of the embryos produced by homozygous Trl[13C] mutant mothers survive to the larval stages, even when fertilised by wild type males. A large proportion of embryos have severe defects in nuclear division during the syncitial blastoderm stage. Approximately 80% of Trl[13C]/Trl[R67] mutant males show a transformation of the sixth abdominal segment towards the fifth. Fewer than 20% of the embryos produced by homozygous Trl[13C]/Trl[R67] mutant mothers survive to the larval stages, even when fertilised by wild type males. (Greenberg and Schedl, 2001) Lethality occurs at hatching. (Katokhin et al., 2001) corto⁴²⁰ Trl^13C double heterozygotes give a higher percentage of postpronotum defects than expected for an additive phenotype, indicating a synergistic interaction. (Lopez et al., 2001) Trl^13C suppresses the pairing-dependent silencing of w^{enh.iab-7PRE.260} seen in flies homozygous for P{iab-7PRE-w^enh.260}1. (Mishra et al., 2001) Heterozygotes show the formation of "granular" chromatin during interphase and mitosis and high proportions of cells (which are arrested at the G2/M boundary) have hypercondensed chromatin. Cells with abnormal chromosome segregation are also seen. Heterozygous females have egg chambers with an abnormal number of trophocytes and defects in chromatin condensation. (Trunova et al., 2001) Mutant heterozygotes show no enhancement or suppression of the eye phenotype see in P{UAS-lacZ.Abd-B.5F24}. (Cavalli, 1999) Trl^13C, w^Fab-7.24F6 mutants show a 2 fold reduction in eye pigment levels compared to Trl⁺ siblings: PcG-mediated silencing. (Cavalli and Paro, 1998) Enhances the position effect variegation at the w locus caused by In(1)w^m4 and enhances the position effect variegation at the bw locus caused by In(2R)bw^VDe2. Does not effect position effect variegation at the bw locus caused by Byron. (Sass and Henikoff, 1998) Pairing sensitive repression is alleviated in iab-7 PRE lines that carry Trl^13C, suppression is stronger when Trl^13C is homozygous. (Hagstrom et al., 1997) A small percentage of homozygous Trl^13C females survive to the adult stage and produce at least some eggs. Embryos can be divided into three classes. Class I arrest prior to cellularization. Class II arrest between stages 11 and 15. Class III survive gastrulation though later display segmentation defects and die by the first larval instar stage. The proportions of embryos falling into these three classes varies. Transheterozygotes with Trl⁶² exhibit a similar range of phenotypes. In eggs from Trl^13C homozygous mothers, the nuclear cycles become asynchronous during the syncytial blastoderm stage. Chromosome segregation during anaphase is abnormal, and chromosome fragmentation is evident. (Bhat et al., 1996) Flies doubly heterozygous for Ubx¹³⁰ and Trl^13C show larger halteres than Ubx¹³⁰ heterozygotes alone. Flies are weakly viable and eclose at 30-40% of expected frequency, but becomes inviable when combined with z mutants. (Laney and Biggin, 1996) Homozygous female escapers can be obtained. (Liaw et al., 1995) Trl^13C+/+Ubx¹³⁰ double heterozygous flies have larger halteres than Ubx¹³⁰ heterozygotes. Homeotic transformations include a low frequency of patches on the haltere and postnotum that develop like wing and notum tissues respectively. (Farkas et al., 1994) Reduced viability and a rough eye phenotype. Homozygous adult males show homeotic transformation of the sixth sternite into the fifth as shown by the presence of a few bristles on the sixth sternite, this phenotype is stronger in hemizygotes but they die just before hatching. In(1)w^m4h males also heterozygous for Trl^13C have almost white eyes, indicating a more frequent inactivation of the w gene. Heterozygotes with Trl⁶² eclose late, are poorly viable and exhibit the same phenotypes as Trl^13C homozygotes. (Farkas et al., 1994)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Position of the first base of the 8-bp target site duplication. (Katokhin et al., 2001, Katokhin et al., 2001) P-element insertion in the transcription unit. The orientation of the insertion was not reported. (Farkas et al., 1994) Insertion origonally mapped to an EcoRI-BamHI fragment at 3L:14746987..14750181 (r5). (Farkas et al., 1994)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{bluetail}Trl^13C (FlyBase, 1992-, Farkas et al., 1994, )
Secondary FlyBase IDs

References ( 22 )

Generate a list of	All references relating to this insertion ( 22 )

List References by type	Research paper ( 20 ) Review ( 1 ) FlyBase analysis ( 1 )
Recent research papers (0)
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Dmel\P{bluetail}Trl13C Insertion

Dmel\P{bluetail}Trl^13C Insertion