Possible off-target effects (low probability).
Targets multiple paralogous genes.
Expression under the control of Scer\GAL4pnr-MD237 may result in lethality, depending on the insertion line used.
Expression of P{GD6142} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies.
Adults expressing P{GD6142} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or late pupal lethality.
Possible off-target effects (low probability).
Targets multiple paralogous genes.
Expression under the control of Scer\GAL4pnr-MD237 may result in lethality, depending on the insertion line used.
Expression of P{GD6142} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies.
Adults expressing P{GD6142} under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or late pupal lethality.