A 3,547 bp genomic DNA fragment containing the complete coding sequence of TbCMF46-RA and TbCMF46-RB from Canton-S starting at 1,484 bp upstream of the TbCMF46-RB ATG is fused in frame to EGFP.
TbCMF46-GFP is observed in embryos in the chordotonal neurons at the base of the cilium, presumably the basal body region, and at the tip of the cilium. It is also detected as a dot in the external sensory organs.