Reference Report

Reference
Citation	Laurençon, A., Dubruille, R., Efimenko, E., Grenier, G., Bissett, R., Cortier, E., Rolland, V., Swoboda, P., Durand, B. (2007). Identification of novel regulatory factor X (RFX) target genes by comparative genomics in Drosophila species. Genome Biol. 8(9): R195. (Export to RIS)
FlyBase ID	FBrf0216119
Publication Type	Research paper
PubMed ID	17875208
PubMed Abstract	Regulatory factor X (RFX) transcription factors play a key role in ciliary assembly in nematode, Drosophila and mouse. Using the tremendous advantages of comparative genomics in closely related species, we identified novel genes regulated by dRFX in Drosophila.We first demonstrate that a subset of known ciliary genes in Caenorhabditis elegans and Drosophila are regulated by dRFX and have a conserved RFX binding site (X-box) in their promoters in two highly divergent Drosophila species. We then designed an X-box consensus sequence and carried out a genome wide computer screen to identify novel genes under RFX control. We found 412 genes that share a conserved X-box upstream of the ATG in both species, with 83 genes presenting a more restricted consensus. We analyzed 25 of these 83 genes, 16 of which are indeed RFX target genes. Two of them have never been described as involved in ciliogenesis. In addition, reporter construct expression analysis revealed that three of the identified genes encode proteins specifically localized in ciliated endings of Drosophila sensory neurons.Our X-box search strategy led to the identification of novel RFX target genes in Drosophila that are involved in sensory ciliogenesis. We also established a highly valuable Drosophila cilia and basal body dataset. These results demonstrate the accuracy of the X-box screen and will be useful for the identification of candidate genes for human ciliopathies, as several human homologs of RFX target genes are known to be involved in diseases, such as Bardet-Biedl syndrome.
DOI	10.1186/gb-2007-8-9-r195
Related Publication(s)
Personal communication to FlyBase	mecC and Oseg1. Avidor-Reiss, 2012.9.12, mecC and Oseg1. [FBrf0219417]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genome Biol.
Title	Genome Biology
Publication Year	2000-
ISBN/ISSN	1465-6906
Data from Reference
Aberrations (1)
	Df(3R)S143702
Alleles (13)
	CG31036^{T:Avic\GFP-EGFP} Disc\RFP^Scer\UAS.cUa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Rfx⁴⁹ Rfx²⁵³ Rootletin^{T:Avic\GFP-EGFP} Scer\GAL4^CG3259.PA-R Scer\GAL4^CG9227.PAR Scer\GAL4^elav-C155 Scer\GAL4^mecC.PA-R Scer\GAL4^osm-1.PA-R T:Avic\GFP^EGFP TbCMF46^{T:Avic\GFP-EGFP}
Constructs (9)
	P{CG3259-GAL4.A} P{CG9227-GAL4.AR} P{CG31036.GFP} P{mecC-GAL4} P{osm-1-GAL4.A} P{Rootletin.GFP} P{TbCMF46.GFP} P{UAS-mCD8::GFP.L} P{UAS-RFP.U}
Genes (59)
	BBS1 BBS4 BBS8 btv Cby CG1126 CG1399 CG2006 CG3259 CG3769 CG4525 CG5142 CG5195 CG5359 CG7735 CG11755 CG13178 CG14367 CG14870 CG15161 CG15666 CG17150 CG17599 CG30441 CG31036 CG31249 CG32392 CG43370 Che-13 Dhc93AB Disc\RFP dnd Efhc1.2 elav eys futsch Hnf4 iav Kap3 king-tubby Klp64D Mmus\Cd8a Nmda1 nompB nudC Oseg1 Oseg4 Oseg5 Oseg6 osm-1 osm-6 Pkd2 pros rempA Rfx Rootletin Scer\GAL4 TbCMF46 tectonic
Insertions (2)
	P{?lArB}Rfx⁴⁹ P{GawB}elav^C155
Natural transposons (1)
	P-element