FB2025_01 , released February 20, 2025
Recombinant Construct: P{UAS-HA-UbAA}
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General Information
Symbol
P{UAS-HA-UbAA}
FlyBase ID
FBtp0126792
Feature type
transgenic_transposable_element
Component Allele(s)
Uuuu\UbAA.UAS.Tag:HA
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Uuuu\UbAA.UAS.Tag:HA
Transgenic product class
missense_variant
(Wang et al., 2017)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Uuuu\Ub
Tagged with
Tag:HA
Description

UAS regulatory sequences drive expression of Ubiquitin containing the amino acid replacements G75A and G76A. The mutant protein can be conjugated by the endogenous ubiquitin C-terminus onto the Lys48 residue, forming free ubiquitin chains, but is unable to conjugate onto substrates including endogenous ubiquitin due to the C-terminal mutations. The protein is tagged with Tag:HA.

(Wang et al., 2017)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-HA-UbAA}
(FlyBase, 1992-)
UAS-HA-UbAA
(Wang et al., 2017)
Secondary FlyBase IDs
    References (2)