FB2025_01 , released February 20, 2025
Recombinant Construct: P{nanosP-mito-roGFP2-Orp1-nanos3'UTR}
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General Information
Symbol
P{nanosP-mito-roGFP2-Orp1-nanos3'UTR}
FlyBase ID
FBtp0134195
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFProGFP2-Orp1.mito.nanos
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFProGFP2-Orp1.mito.nanos
Transgenic product class
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
roGFP2-Orp1
Tagged with
Tag:Mito(hCOX8A)
Description

A 935bp nos promoter drives expression of the roGFP2-Orp1 fluorescent hydrogen peroxide biosensor. The Tag:Mito(hCOX8A) tag is also present, to target the sensor to the mitochondrion. A 1236bp nos 3'UTR fragment (that stabilizes transcripts in the germline) is present downstream of the coding sequence.

(Nilangekar et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{nanosP-mito-roGFP2-Orp1-nanos3'UTR}
(FlyBase, 1996-)
P{nosP-mito-roGFP2-Orp1-nos3'UTR}
(FlyBase, 1992-)
nosP-mito-roGFP2-Orp1-nos3'UTR
(Nilangekar et al., 2019)
Secondary FlyBase IDs
    References (3)