A neuroblast specific promoter (the 250bp '2-5' sub-fragment from the GMR9D11 enhancer, with mutations of the klu and dpn/E(spl)mγ-HLH protein binding sites) is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by an N-terminal fragment of the E(spl)mγ-HLH coding sequence (includes the destruction sequence, the basic helix-loop-helix, and the Orange motif), tagged at the C-terminal end with Tag:V5. The coding sequence is followed by a synthetic 3'UTR containing 6 BRE motifs (mutated) and 4 ARE motifs (mutated).