FlyBase Allele Report: Dmel\hth[hs.PP]

General Information

Symbol

Dmel\hth^hs.PP

Species

D. melanogaster

Name

heat shock construct of Pai

FlyBase ID

FBal0089222

Feature type

allele

Associated gene

Dmel\hth

Associated Insertion(s)

Carried in Construct

P{hs-hth.P}

Also Known As

hs-hth

Key Links

Transgenic product class

cDNA

(Pai et al., 1998)

wild_type

(Pai et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Pai et al., 1998)

wild_type

(Pai et al., 1998)

Mutagen

in vitro construct

(Pai et al., 1998)

Progenitor genotype

Carried in construct

P{hs-hth.P}

Cytology

Description

A 1.6kb hth cDNA fragment containing the entire open reading frame is expressed under the control of an Hsp70 promoter.

(Pai et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

hth

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive

(Pai et al., 1998)

visible | heat sensitive

(Pai et al., 1998)

Phenotype Manifest In

leg | heat sensitive

(Pai et al., 1998)

tarsal segment | heat sensitive

(Pai et al., 1998)

tibia | heat sensitive

(Pai et al., 1998)

Detailed Description

Statement

Reference

Expression of hth^hs.PP in a wild-type background using a 2 hour heat shock during embryogenesis causes significant lethality, with the effect being strongest 0-4 and 4-8 hours after egg laying. The few flies that survive to adulthood are morphologically normal. Ectopic expression of hth^hs.PP during the third larval instar and early pupal stages results in severe shortening of the legs, due to a lack or shortening of the tibia and tarsal segments.

(Pai et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

hth^hs.PP partially rescues hth^dtl-S142204

(Pai et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hth^hs.PP

Name Synonyms

heat shock construct of Pai

(Pai et al., 1998)

Secondary FlyBase IDs

References (3)

Report Sections

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