FB2025_01 , released February 20, 2025
Allele: Dmel\kek1::kek2L.UAS.GFP
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General Information
Symbol
Dmel\kek1::kek2L.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0155175
Feature type
allele
Associated gene
kek1::kek2
Associated Insertion(s)
Carried in Construct
P{UAS-kek1::kek2.LGFP}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Alvarado et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-kek1::kek2.LGFP}
Cytology
Description

UAS regulatory sequences drive expression of a chimeric construct in which part of kek1 has been replaced with the corresponding region of kek2. The chimeric protein consists of the LRRs (including the cysteine-rich flanks) of kek1 and the Ig, juxta/transmembrane and cytoplasmic regions of kek2 and is tagged at the C-terminal end with GFP.

(Alvarado et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
kek1::kek2
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of kek1::kek2L.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4CY2 results in eggs with either wild type or slightly ventralised chorions. Expression of kek1::kek2L.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PF results in eyes which are either wild type or have a slightly rough phenotype. Expression of kek1::kek2L.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4Act5C.PI results in about 30% lethality.

      (Alvarado et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      kek1::kek2L.Scer\UAS.T:Avic\GFP
      kek1::kek2L.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)