FB2025_01 , released February 20, 2025
Allele: Dmel\wtrwDN.UAS
Open Close
General Information
Symbol
Dmel\wtrwDN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0216275
Feature type
allele
Associated gene
Dmel\wtrw
Associated Insertion(s)
Carried in Construct
P{UAS-wtrw.DN}
Key Links
Transgenic product class
dominant_negative_variant
(Liu et al., 2007)
inframe_deletion
(Liu et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Liu et al., 2007)
inframe_deletion
(Liu et al., 2007)
Mutagen
in vitro construct
(Liu et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-wtrw.DN}
Cytology
Description

UASt regulatory sequences drive expression of the amino terminal portion (including the first predicted transmembrane domain) of wtrw. Encodes a dominant negative form of wtrw.

(Liu et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
wtrw
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing wtrwDN.Scer\UAS under the control of one of Scer\GAL4elav.PLu or Scer\GAL4wtrw.PL show disrupted hygrosensing behaviour; a higher fraction of flies choose moist air (100% humidity) over dry air (0% humidity) compared to wild-type controls in a hygrosensory behaviour assay after five minutes.

      Flies expressing wtrwDN.Scer\UAS under the control of Scer\GAL4wtrw.PL show an impaired electrophysiological response to moist air, while leaving dry air sensing unaffected (recordings were taken at the base of the 2nd antennal segment in these experiments).

      (Liu et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      wtrwDN.Scer\UAS
      wtrwDN.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)