Allele Dmel\sas-6^{Ubi.P.T:Avic\GFP}

General Information
Symbol	Dmel\sas-6^{Ubi.P.T:Avic\GFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0240462
Feature type	allele	Associated gene	Dmel\sas-6

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Peel et al., 2007) in vitro construct - regulatory fusion (Peel et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A ubiquitin promoter drives expression of sas-6 which is tagged with T:Avic\GFP. (Peel et al., 2007)
Carried in construct	P{Ubi-GFP-sas-6.P} (Dix and Raff, 2007, Peel et al., 2007, Stevens et al., 2010)
Tagged with	T:Avic\GFP (Peel et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	centriole (Peel et al., 2007, Stevens et al., 2010) centriole \| ectopic (Peel et al., 2007)
Detailed Description
	Statement Reference Unfertilized eggs laid by mothers carrying two copies of sas-6[Ubi.P.T:Avic\GFP] assemble centriole-like structures. (Stevens et al., 2010) Approximately 3% of centrioles proceed through an extra round of template-driven replication within a single nuclear cycle in syncytial embryos expressing sas-6[Ubi.P.T:Avic\GFP], and a few centrioles undergo two extra rounds of replication. Extra centrioles are detected in mitotic third larval instar brain cells in animals expressing sas-6[Ubi.P.T:Avic\GFP]. The brain cells do not have dramatic mitotic defects, and the accumulation of extra centrioles is not due to a failure in cytokinesis. No extra centrioles are detected in the female or male germline in animals expressing sas-6[Ubi.P.T:Avic\GFP]. Expression of sas-6[Ubi.P.T:Avic\GFP] results in de novo formation of centriole-like structures in approximately 30% of unfertilised eggs (which do not normally contain centrioles). (Peel et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference sas-6^{Ubi.P.T:Avic\GFP} has centriole phenotype, enhanceable by ana2^{Ubi.C.T:Avic\GFP} (Stevens et al., 2010)
NOT Enhanced by
Statement Reference sas-6^{Ubi.P.T:Avic\GFP} has centriole phenotype, non-enhanceable by SAK^{Ubi.P.T:Avic\GFP} (Stevens et al., 2010) sas-6^{Ubi.P.T:Avic\GFP} has centriole phenotype, non-enhanceable by Sas-4^{Ubi.P.T:Avic\GFP} (Stevens et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Almost all unfertilized eggs laid by mothers carrying one copy of sas-6[Ubi.P.T:Avic\GFP] and ana2[Ubi.C.T:Avic\GFP] contain hundreds of large structures that stain for centriole markers, recruited PCM, and nucleated asters. These centriole-like structures often appear ring-shaped, and only one structure is contained within each aster. Eggs laid by mothers carrying one copy of either ana2[Ubi.C.T:Avic\GFP] or sas-6[Ubi.P.T:Avic\GFP] together with one copy of either SAK[Ubi.P.T:Avic\GFP] or Sas-4[Ubi.P.T:Avic\GFP] exhibit at most a very small number of asters in very few eggs. (Stevens et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	sas-6^{Ubi.P.T:Avic\GFP} rescues sas-6^c02901 (Peel et al., 2007)
Comments	The uncoordinated phenotype of sas-6[c02901] adults is rescued by sas-6[Ubi.P.T:Avic\GFP]. (Peel et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	sas-6^{Ubi.P.T:Avic\GFP}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Stevens et al., 2010, J. Cell Biol. 188(3): 313--323 Drosophila Ana2 is a conserved centriole duplication factor. [FBrf0209887] Peel et al., 2007, Curr. Biol. 17(10): 834--843 Overexpressing centriole-replication proteins in vivo induces centriole overduplication and de novo formation. [FBrf0201218]
Supplementary material	Dix and Raff, 2007, Curr. Biol. 17(20): Document S1. Experimental Procedures and Two Figures. [FBrf0209277]

Allele Dmel\sas-6Ubi.P.T:Avic\GFP

Allele Dmel\sas-6^{Ubi.P.T:Avic\GFP}