FlyBase Allele Report: Dmel\Chro[612]

General Information

Symbol

Dmel\Chro⁶¹²

Species

D. melanogaster

Name

FlyBase ID

FBal0243233

Feature type

allele

Associated gene

Dmel\Chro

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Rath et al., 2006)

Progenitor genotype

Cytology

Description

Truncated Chro⁶¹² protein is missing parts of the COOH-terminal domain important for spindle localisation.

(Ding et al., 2009)

EMS-generated point mutation in Chro that introduces a premature stop codon resulting in a truncated 612 amino acid protein that retains the chromodomain.

(Rath et al., 2006)

Nucleotide substitution: C2024T

(Rath et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

imaginal disc | third instar larval stage (with Chro⁷¹)

(Ding et al., 2009)

larval brain | third instar larval stage (with Chro⁷¹)

(Ding et al., 2009)

larval neuroblast | third instar larval stage (with Chro⁷¹)

(Ding et al., 2009)

neuroblast | third instar larval stage (with Chro⁷¹)

(Li et al., 2017)

neuroblast | third instar larval stage (with Df(3L)ED231)

(Li et al., 2017)

polytene chromosome (with Chro⁷¹)

(Yao et al., 2012)

spindle | third instar larval stage (with Chro⁷¹)

(Ding et al., 2009)

Detailed Description

Statement

Reference

In contrast to wild-type, in Chro⁷¹/Chro⁶¹² mutant larvae at 96h after larval hatching (ALH) 26% of mira-positive central brain neural stem cells (NSCs) retain their cellular processes and number of actively dividing dpn-positive NSCs is much reduced compared to controls. Chro⁶¹²/Df(3L)ED231 mutants display similar NSC reactivation defects.

(Li et al., 2017)

Polytene chromosomes of Chro⁷¹/Chro⁶¹² third instar larvae show disruptions of the band/interband regions and the chromosome arms are coiled and condensed.

(Yao et al., 2012)

90% of Chro⁷¹/Chro⁶¹² mutant larval neuroblasts have severe spindle and chromosome segregation defects. The microtubule spindles are incomplete, unfocussed, and/or without clear spindle poles. At anaphase, chromosomes are lagging and scattered.

The mitotic index is significantly reduced in Chro⁷¹/Chro⁶¹² mutants, and an associated small brain and imaginal disc phenotype is observed.

(Ding et al., 2009)

Chro⁷¹/Chro⁶¹² transheterozygotes survive to third instar larval stage but no pupae are observed.

Polytene chromosome morphology is disturbed in Chro⁷¹/Chro⁶¹² mutant third instar larvae.

(Rath et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Chro⁷¹/Chro⁶¹² has lethal | third instar larval stage phenotype, enhanceable by JIL-1^z2

(Rath et al., 2006)

Suppressed by

Statement

Reference

Chro⁷¹/Chro⁶¹² has decreased occurrence of cell division | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^insc-Mz1407/grh^UAS.cUa

(Li et al., 2017)

Chro⁷¹/Chro⁶¹² has decreased occurrence of cell division | third instar larval stage phenotype, suppressible | partially by pros[+]/pros¹⁷

(Li et al., 2017)

Chro⁷¹/Chro⁶¹², pros[+]/pros¹⁷ has decreased occurrence of cell division | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^insc-Mz1407/grh^UAS.cUa

(Li et al., 2017)

Chro⁷¹/Chro⁶¹², Scer\GAL4^insc-Mz1407, grh^UAS.cUa has decreased occurrence of cell division | third instar larval stage phenotype, suppressible | partially by pros[+]/pros¹⁷

(Li et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Chro⁷¹/Chro⁶¹² has neuroblast | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^insc-Mz1407/grh^UAS.cUa

(Li et al., 2017)

Chro⁷¹/Chro⁶¹² has neuroblast | third instar larval stage phenotype, suppressible | partially by pros[+]/pros¹⁷

(Li et al., 2017)

Chro⁷¹/Chro⁶¹², pros[+]/pros¹⁷ has neuroblast | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^insc-Mz1407/grh^UAS.cUa

(Li et al., 2017)

Chro⁷¹/Chro⁶¹², Scer\GAL4^insc-Mz1407, grh^UAS.cUa has neuroblast | third instar larval stage phenotype, suppressible | partially by pros[+]/pros¹⁷

(Li et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The central brain neuroblast reactivation defects (manifested by the retention of cellular extensions and low mitotic activity even at late larval stages) characteristic for Chro⁷¹/Chro⁶¹² mutants are alleviated by expression of grh^Scer\UAS.cUa under the control of Scer\GAL4^insc-Mz1407 in the mutant background or by combination with a single copy of pros¹⁷, the phenotype can be further improved when both grh^Scer\UAS.cUa expression as well as the pros¹⁷ heterozygosity are combined.

(Li et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Chro⁷¹/Chro⁶¹² is rescued by Chro^{+tCH322-159M1}

(Li et al., 2017)

Df(3L)ED231/Chro⁶¹² is rescued by Chro^{+tCH322-159M1}

(Li et al., 2017)

Chro⁷¹/Chro⁶¹² is rescued by Scer\GAL4^unspecified/Chro^{UASp.FL.Tag:HA,Tag:FLAG,GFP}

(Yao et al., 2012)

Partially rescued by

Chro⁷¹/Chro⁶¹² is partially rescued by Scer\GAL4^unspecified/Chro^{NTD.UASp.GFP.Tag:NLS(SV40-largeT)}

(Yao et al., 2012)

Chro⁷¹/Chro⁶¹² is partially rescued by Scer\GAL4^elav-C155/Chro^GFP

(Ding et al., 2009)

Not rescued by

Chro⁷¹/Chro⁶¹² is not rescued by Scer\GAL4^unspecified/Chro^{NTD-ΔChD.UAS.Tag:NLS(Unk),Tag:V5}

(Yao et al., 2012)

Chro⁷¹/Chro⁶¹² is not rescued by Scer\GAL4^unspecified/Chro^{ChD.UAS.Tag:NLS(SV40-largeT),Tag:V5,GFP}

(Yao et al., 2012)

Chro⁷¹/Chro⁶¹² is not rescued by Scer\GAL4^unspecified/Chro^CTD.UASp.GFP

(Yao et al., 2012)

Comments

The brain neuroblast reactivation defects observed in either Chro⁷¹/Chro⁶¹² or Chro⁶¹²/Df(3L)ED231 mutant larvae at 96h after larval hatching can be rescued by combination with Chro^{+tCH322-159M1}.

(Li et al., 2017)

Expression of Chro^{Scer\UAS.P\T.FL.T:Ivir\HA1,T:Zzzz\FLAG,T:Avic\GFP} in third larval instar salivary glands rescues all aspects of polytene chromosome morphology in Chro⁷¹/Chro⁶¹² animals.

Expression of Chro^{NTD.Scer\UAS.P\T.T:Avic\GFP.T:SV40\nls2} in third larval instar salivary glands substantially rescues polytene chromosome morphology in Chro⁷¹/Chro⁶¹² animals, although some regions of the chromosome arms remain coiled.

Expression of either Chro^{NTD-ΔChD.Scer\UAS.T:Uuuu\nls6,T:SV5\V5} or Chro^{ChD.Scer\UAS.T:SV40\nls2,T:SV5\V5,T:Avic\GFP} in third larval instar salivary glands results in no or very little improvement of polytene chromosome morphology in Chro⁷¹/Chro⁶¹² animals.

Expression of Chro^{CTD.Scer\UAS.P\T.T:Avic\GFP} in third larval instar salivary glands does not rescue polytene chromosome morphology in Chro⁷¹/Chro⁶¹² animals.

(Yao et al., 2012)

Expression of Chro^{Scer\UAS.P\T.T:Avic\GFP} with Scer\GAL4^elav-C155 partially rescues the smaller brain size, and coiled and condensed chromosome arm morphology of the polytene chromosomes from Chro⁷¹/Chro⁶¹² mutant larvae.

(Ding et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93148

w^*; Chro⁶¹²/TM6C, Sb¹ Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Chro⁶¹²

(Yao et al., 2012, Ding et al., 2009, Rath et al., 2006)

chro⁶¹²

(Li et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close