FB2025_01 , released February 20, 2025
Allele: Scer\GAL4Gr89a.2
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General Information
Symbol
Scer\GAL4Gr89a.2
Species
S. cerevisiae
Name
FlyBase ID
FBal0249513
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Gr89a-GAL4.2}
Also Known As
Gr89a-GAL4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Weiss et al., 2011)
Progenitor genotype
Carried in construct
P{Gr89a-GAL4.2}
Cytology
Description

A 2kb Gr89a promoter fragment is fused upstream of sequence encoding a GAL4 driver.

(Weiss et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
Gr89a
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      abdominal 1 ventral multidendritic neuron v'td2
      (Qian et al., 2018)
      abdominal 2 ventral multidendritic neuron v'td2
      (Qian et al., 2018)
      abdominal 3 ventral multidendritic neuron v'td2
      (Qian et al., 2018)
      abdominal 4 ventral multidendritic neuron v'td2
      (Qian et al., 2018)
      abdominal 5 ventral multidendritic neuron v'td2
      (Qian et al., 2018)
      abdominal 6 ventral multidendritic neuron v'td2
      (Qian et al., 2018)
      adult stage
      high salt/bitter-sensing neuron | subset of prothoracic tarsal segment 5
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of prothoracic tarsal segment 4
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of mesothoracic tarsal segment 5
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of mesothoracic tarsal segment 4
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of metathoracic tarsal segment 5
      (Ling et al., 2014)
      high salt/bitter-sensing neuron | subset of metathoracic tarsal segment 4
      (Ling et al., 2014)
      gustatory receptor neuron | subset of short labellar taste bristle | subset
      (Weiss et al., 2011)
      gustatory receptor neuron | subset of intermediate labellar taste bristle | subset
      (Weiss et al., 2011)
      high salt/bitter-sensing neuron of adult labellum sensillum
      (Weiss et al., 2011)
      bitter-sensitive labellar taste bristle
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-a
      (Weiss et al., 2011)
      intermediate labellar taste bristle
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-b
      (Weiss et al., 2011)
      short labellar taste bristle S-b
      (Weiss et al., 2011)
      short labellar taste bristle S-a | intense
      (Weiss et al., 2011)
      bitter-sensitive labellar taste bristle | intense
      (Weiss et al., 2011)
      short labellar taste bristle S-b | intense
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-a | intense
      (Weiss et al., 2011)
      intermediate labellar taste bristle | intense
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-b | intense
      (Weiss et al., 2011)
      adult abdominal multidendritic neuron | subset
      (Park and Kwon, 2011)
      short labellar taste bristle S-a
      (Weiss et al., 2011)
      bitter-sensing neuron | subset
      (Israel et al., 2022)
      Additional Information
      Statement
      Reference

      ScerGAL4Gr89a.2 drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Additionally, expression is driven in laterally localized multidendritic neurons with extensive arborization. Expression is also observed in projections to the abdominal ganglion.

      (Park and Kwon, 2011)

      ScerGAL4Gr89a.2 is expressed in gustatory neurons of all of the bitter-sensitive short labellar taste bristles, and in gustatory neurons of all of the intermediate labellar taste bristles.

      (Weiss et al., 2011)

      ScerGAL4Gr89a.2 is strongly expressed in almost all of the short and intermediate taste sensilla of the labellum.

      (Weiss et al., 2011, Weiss et al., 2011)
       
      Marker for
      bitter-sensing neuron
      (Israel et al., 2022)
      high salt/bitter-sensing neuron
      (Ganguly et al., 2017)
      Reflects expression of
      Gr89a
      (Israel et al., 2022, Ling et al., 2014, Park and Kwon, 2011, Weiss et al., 2011, Weiss et al., 2011)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 32[o]C) of Scer\GAL4Gr89a.2 neurons induces some proboscis extension, compared to controls, but this is at a low frequency.

      (Kain and Dahanukar, 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Ir56bUAS.cDa, Scer\GAL4Gr89a.2 has abnormal neurophysiology | adult stage phenotype, suppressible by Ir76b1

      (Dweck et al., 2022)
      Enhancer of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Gr89a.2
      Name Synonyms
      Secondary FlyBase IDs
        References (18)