FB2025_01 , released February 20, 2025
Allele: Hsap\APPT12.Aβ40.UAS.Tag:SS(nec)
Open Close
General Information
Symbol
Hsap\APPT12.Aβ40.UAS.Tag:SS(nec)
Species
H. sapiens
Name
FlyBase ID
FBal0276870
Feature type
allele
Associated gene
Hsap\APP
Associated Insertion(s)
Carried in Construct
M{UAS-T12-Aβ40}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Speretta et al., 2012)
Progenitor genotype
Carried in construct
M{UAS-T12-Aβ40}
Cytology
Description

UASt regulatory sequences drive expression of the Tag:SS(nec) secretory signal peptide followed by two tandem copies of the Aβ (amyloid β) monomer linked together by a 12 amino acid linker peptide. Both copies of Aβ are the 40 amino acid residue form.

(Speretta et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\APP
Tagged with
Tag:SS(nec)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Alzheimer's disease
      CEA
      (Speretta et al., 2012)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Locomotor rhythm in constant darkness (DD) is comparable to controls when Hsap\APPT12.Aβ40.Scer\UAS is expressed under the control of Scer\GAL4tim.PE or Scer\GAL4P2.4.Pdf.

      (Chen et al., 2014)

      Flies expressing Hsap\APPT12.Aβ40.Scer\UAS under the control of Scer\GAL4GMR.PF form significant quantities of large, SDS-insoluble deposits in the head. Abundant SDS-soluble oligomeric Aβ aggregates are also present. No detectable SDS-soluble oligomeric Aβ aggregates are present.

      Expression of Hsap\APPT12.Aβ40.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in a detectable rough eye phenotype.

      Flies expressing one copy of Hsap\APPT12.Aβ40.Scer\UAS under the control of Scer\GAL4elav-C155 do not have a significantly different life span compared to wild-type controls.

      (Speretta et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\APPT12.Aβ40.Scer\UAS.T:SS-nec
      Hsap\APPT12.Aβ40.Scer\UAS
      Hsap\APPT12.Aβ40.UAS.Tag:SS(nec)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)