FB2025_01 , released February 20, 2025
Allele: Hsap\APPT22.Aβ42.UAS.Tag:SS(nec)
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General Information
Symbol
Hsap\APPT22.Aβ42.UAS.Tag:SS(nec)
Species
H. sapiens
Name
FlyBase ID
FBal0276874
Feature type
allele
Associated gene
Hsap\APP
Associated Insertion(s)
Carried in Construct
M{UAS-T22-Aβ42}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Speretta et al., 2012)
Progenitor genotype
Carried in construct
M{UAS-T22-Aβ42}
Cytology
Description

UASt regulatory sequences drive expression of the Tag:SS(nec) secretory signal peptide followed by two tandem copies of the Aβ (amyloid β) monomer linked together by a 22 amino acid linker peptide. Both copies of Aβ are the 42 amino acid residue form.

(Speretta et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\APP
Tagged with
Tag:SS(nec)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Hsap\APPT22.Aβ42.Scer\UAS under the control of Scer\GAL4GMR.PF form significant quantities of large, SDS-insoluble deposits in the head.

      Flies expressing Hsap\APPT22.Aβ42.Scer\UAS under the control of Scer\GAL4GMR.PF have severe eye malformation with loss of the ommatidial arrays and the appearance of melanised patches of necrotic tissue.

      Flies expressing one copy of Hsap\APPT22.Aβ42.Scer\UAS under the control of Scer\GAL4elav-C155 have a significantly shorter lifespan than wild-type controls and than flies expressing two copies of Hsap\APPAβ42.Scer\UAS.att under the control of Scer\GAL4elav-C155.

      (Speretta et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\APPT22.Aβ42.Scer\UAS.T:SS-nec
      Hsap\APPT22.Aβ42.Scer\UAS
      Hsap\APPT22.Aβ42.UAS.Tag:SS(nec)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)