FlyBase Allele Report: Dmel\Ccdc56[D11]

General Information

Symbol

Dmel\Ccdc56^D11

Species

D. melanogaster

Name

FlyBase ID

FBal0277037

Feature type

allele

Associated gene

Dmel\Ccdc56

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Peralta et al., 2012)

Mutagen

P-element activity

(Peralta et al., 2012)

Progenitor genotype

P{SUPor-P}CG11658^KG07792

(Peralta et al., 2012)

Cytology

Description

Excision of the P{SUPor-P} element from P{SUPor-P}KG07792 results in a 1168bp deletion that comprises the entire Ccdc56 coding region and the first 26 nucleotides of the intron in the Ccdc56-mtTFB1 mRNA.

(Peralta et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:11,708,206..11,709,373 [+]

Comment:

1168 bp deletion resulting from the imprecise excision of P{SUPor-P}CG11658^KG07792, which includes the entire coding region of Ccdc56 ORF and 26 bases of the intron of the dicistronic transcript..

(Peralta et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing disc | third instar larval stage

(Peralta et al., 2012)

Detailed Description

Statement

Reference

Analysis of mouth hook morphology shows that homozygous Ccdc56^D11 mutants take 10-12 days to reach the third instar larval stage, compared to 4-5 days in controls. The resulting third instar larvae are smaller than controls, remain at this stage for over 20 days, and die before pupariation.

Homozygous Ccdc56^D11 mutant imaginal discs are smaller than in controls, showing a reduction in the number of mitotic cells and increased levels of apoptosis.

(Peralta et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ccdc56^D11 has lethal | recessive | third instar larval stage phenotype, suppressible | partially by mtTFB1^bi.UASp/Scer\GAL4^arm.PU

(Peralta et al., 2012)

NOT suppressed by

Statement

Reference

Ccdc56^D11 has lethal | recessive | third instar larval stage phenotype, non-suppressible by mtTFB1^UASp.cPa/Scer\GAL4^arm.PU

(Peralta et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of mtTFB1^{Scer\UAS.P\T.cPa} under the control of Scer\GAL4^arm.PU fails to suppress the larval lethality seen in Ccdc56^D11.

Expression of mtTFB1^{bi.Scer\UAS.P\T} under the control of Scer\GAL4^arm.PU partially suppresses the lethality seen in Ccdc56^D11 mutants. Approximately 97% of mutant progeny reach the pupal stage and around 35% reach the adult stage.

(Peralta et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Ccdc56^D11 is partially rescued by Ccdc56^UASp.cPa/Scer\GAL4^arm.PU

(Peralta et al., 2012)

Ccdc56^D11 is partially rescued by Ccdc56^bi.UASp/Scer\GAL4^arm.PU

(Peralta et al., 2012)

Comments

Ccdc56^D6 fails to complement the lethality seen in Ccdc56^D11.

Expression of Ccdc56^{bi.Scer\UAS.P\T} under the control of Scer\GAL4^arm.PU partially rescues the lethality seen in Ccdc56^D11 mutants. Approximately 97% of mutant progeny reach the pupal stage and around 35% reach the adult stage.

(Peralta et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ccdc56^D11

ccdc56^D11

(Peralta et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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