FlyBase Allele Report: Hsap\DAPK1[K42A.UAS]

General Information

Symbol

Hsap\DAPK1^K42A.UAS

Species

H. sapiens

Name

FlyBase ID

FBal0284426

Feature type

allele

Associated gene

Hsap\DAPK1

Associated Insertion(s)

Carried in Construct

P{UAS-hDAPK1.K42A}

Key Links

Transgenic product class

missense_variant

(Wu et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Wu et al., 2011)

Mutagen

in vitro construct

(Wu et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-hDAPK1.K42A}

Cytology

Description

Amino acid replacement: K42A.

(Wu et al., 2011)

UAS regulatory sequences drive expression of mutated Hsap\DAPK1 coding sequences.

(Wu et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\DAPK1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Wu et al., 2011)

ommatidium, with Scer\GAL4^GMR.PF

(Wu et al., 2011)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Wu et al., 2011)

Detailed Description

Statement

Reference

Expression of Hsap\DAPK1^{K42A.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in a severe rough-eye phenotype with loss of ommatidia and disorganisation of the ommatidial array. Photoreceptor neurons are completely absent in these flies.

(Wu et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\DAPK1^K42A.UAS has eye phenotype, enhanceable by Scer\GAL4^GMR.PF/par-1^UAS.cSa

(Wu et al., 2011)

Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

Suppressed by

Statement

Reference

Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by par-1^Δ-16/par-1[+]

(Wu et al., 2011)

Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by par-1^Δ-16/par-1[+]

(Wu et al., 2011)

Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF has photoreceptor neuron phenotype, suppressible | partially by par-1^Δ-16/par-1[+]

(Wu et al., 2011)

Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hsap\MAPT^RNAi.UAS, Scer\GAL4^GMR.PF

(Wu et al., 2011)

Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by Hsap\MAPT^RNAi.UAS, Scer\GAL4^GMR.PF

(Wu et al., 2011)

Enhancer of

Statement

Reference

Hsap\DAPK1^K42A.UAS/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of par-1^UAS.cSa

(Wu et al., 2011)

Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, par-1^UAS.cSa

(Wu et al., 2011)

Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

Hsap\DAPK1^K42A.UAS, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Wu et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

A par-1^Δ-16 heterozygous background alleviates the rough-eye phenotype seen upon expression of Hsap\DAPK1^{K42A.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF. Photoreceptor neurons are partially rescued in these flies, albeit with abnormal numbers and arrangements.

Coexpression of a low-expression Hsap\DAPK1^{K42A.Scer\UAS} with par-1^Scer\UAS.cSa in the developing eye, both under the control of Scer\GAL4^GMR.PF, produces a synergistic interaction, resulting in markedly reduced eye size with fewer ommatidia.

Knockdown of Hsap\MAPT through expression of Hsap\MAPT^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF alleviates the rough-eye phenotype seen upon expression of Hsap\DAPK1^{K42A.Scer\UAS}.

Coexpression of Hsap\DAPK1^{K42A.Scer\UAS} in the developing eye under the control of Scer\GAL4^GMR.PF dramatically enhances Hsap\MAPT^Scer\UAS.cWa toxicity.

(Wu et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\DAPK1^{K42A.Scer\UAS}

Hsap\DAPK1^K42A.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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