FB2025_01 , released February 20, 2025
Allele: Dmel\Tcs5T221A.UAS.Tag:FLAG
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General Information
Symbol
Dmel\Tcs5T221A.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0284612
Feature type
allele
Associated gene
Dmel\Tcs5
Associated Insertion(s)
Carried in Construct
P{UAS-FLAG-Tcs5.T221A}
Key Links
Transgenic product class
missense_variant
(Ibar et al., 2013)
polypeptide_post_translational_processing_variant
(Ibar et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Ibar et al., 2013)
polypeptide_post_translational_processing_variant
(Ibar et al., 2013)
Mutagen
in vitro construct
(Ibar et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-FLAG-Tcs5.T221A}
Cytology
Description

UAS regulatory sequences drive expression of Prpk which contains the amino acid replacement T221A (lacks the Akt1 phosphorylation site). The protein is tagged with Tag:FLAG.

(Ibar et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Tcs5
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of PrpkT221A.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4salm.EPv has no significant effect on wing size.

      (Ibar et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4salm.EPv/Tcs5T221A.UAS.Tag:FLAG rescues Tcs5RNAi.UAS.462

      (Ibar et al., 2013)
      Comments

      Co-expression of PrpkT221A.Scer\UAS.T:Zzzz\FLAG rescues the reduction in cell size seen in the wings of animals expressing PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4salm.EPv.

      (Ibar et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      PrpkT221A.Scer\UAS.T:Zzzz\FLAG
      PrpkT221A.UAS.Tag:FLAG
      PrpkT221A
      (Ibar et al., 2013)
      Tcs5T221A.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)