FlyBase Allele Report: Dmel\ND-23[KK102085]

General Information

Symbol

Dmel\ND-23^KK102085

Species

D. melanogaster

Name

FlyBase ID

FBal0301524

Feature type

allele

Associated gene

Dmel\ND-23

Associated Insertion(s)

Carried in Construct

P{KK102085}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Meadows, 2014.12.12)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Meadows, 2014.12.12)

Mutagen

in vitro construct

(Meadows, 2014.12.12)

Progenitor genotype

Carried in construct

P{KK102085}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Meadows, 2014.12.12)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK102085

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Leigh disease

CEA

(Cabirol-Pol et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Leigh disease

is NOT ameliorated by Pfk^UAS.cTa

(Cabirol-Pol et al., 2018)

is ameliorated by Hsap\SLC2A3^UAS.cBa

(Cabirol-Pol et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain, with Scer\GAL4^repo.PU

(Cabirol-Pol et al., 2018)

adult CNS glial cell, with Scer\GAL4^repo.PU

(Cabirol-Pol et al., 2018)

lipid droplet | adult stage, with Scer\GAL4^repo.PU

(Cabirol-Pol et al., 2018)

ommatidium | progressive, with Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

photoreceptor neuron | progressive, with Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

retina | progressive, with Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

Detailed Description

Statement

Reference

Adults expressing ND-23^KK102085 under the control of Scer\GAL4^elav.PU show severe decreases in lifespan and in locomotor capacity in negative geotaxis assays, and show progressive ommatidium defects (no defects in day 1, decreased number of photoreceptors in day 4 and hardly distinguishable photoreceptors by day 12), and progressive vacuolization of the retina (large vacuoles are detected by day 20, but not by day 4), but do not exhibit obvious synaptic network defects downstream of photoreceptors or brain abnormalities, as compared to controls. Adults expressing ND-23^KK102085 under the control of Scer\GAL4^repo.PU show no decrease in lifespan or in locomotor capacity in negative geotaxis assays, but display massive vacuolization throughout the cortical regions of the brain and optic lobes by day 4, as well as severe increases in the number and size of lipid droplets (assessed by Nile Red or Bodipy staining or EYFP-perilipin-2 fluorescence) in glial cells at the border between the cortex and the neuropile, as compared to controls.

(Cabirol-Pol et al., 2018)

Flies expressing Jafrac1^Scer\UAS.cHa in neurons under the control of Scer\GAL4^elav-C155 show a dramatic reduction in lifespan compared with wild-type.

(Besson et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ND-23^KK102085, Scer\GAL4^elav.PU has short lived phenotype, suppressible | partially by Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

ND-23^KK102085, Scer\GAL4^elav.PU has decreased cell number | adult stage | progressive phenotype, suppressible | partially by Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

ND-23^KK102085, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav-C155

(Besson et al., 2015)

ND-23^KK102085, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Pfk^UAS.cTa, Scer\GAL4^elav-C155

(Besson et al., 2015)

ND-23^KK102085, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by G6pd^UAS.cLa, Scer\GAL4^elav-C155

(Besson et al., 2015)

NOT suppressed by

Statement

Reference

ND-23^KK102085, Scer\GAL4^elav.PU has short lived phenotype, non-suppressible by Pfk^UAS.cTa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

ND-23^KK102085, Scer\GAL4^elav.PU has abnormal neuroanatomy | adult stage | progressive phenotype, non-suppressible by Pfk^UAS.cTa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

ND-23^KK102085, Scer\GAL4^elav.PU has decreased cell number | adult stage | progressive phenotype, non-suppressible by Pfk^UAS.cTa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

ND-23^KK102085, Scer\GAL4^elav.PU has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

ND-23^KK102085, Scer\GAL4^elav.PU has ommatidium | progressive phenotype, suppressible | partially by Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

ND-23^KK102085, Scer\GAL4^elav.PU has photoreceptor neuron | progressive phenotype, suppressible | partially by Hsap\SLC2A3^UAS.cBa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

NOT suppressed by

Statement

Reference

ND-23^KK102085, Scer\GAL4^elav.PU has ommatidium | progressive phenotype, non-suppressible by Pfk^UAS.cTa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

ND-23^KK102085, Scer\GAL4^elav.PU has photoreceptor neuron | progressive phenotype, non-suppressible by Pfk^UAS.cTa, Scer\GAL4^elav.PU

(Cabirol-Pol et al., 2018)

ND-23^KK102085, Scer\GAL4^repo.PU has adult brain phenotype, non-suppressible by Pfk^UAS.cTa, Scer\GAL4^repo.PU

(Cabirol-Pol et al., 2018)

ND-23^KK102085, Scer\GAL4^repo.PU has lipid droplet | adult stage phenotype, non-suppressible by Pfk^UAS.cTa, Scer\GAL4^repo.PU

(Cabirol-Pol et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased lifespan and the ommatidium defects observed in adults expressing ND-23^KK102085 under the control of Scer\GAL4^elav.PU, as well as the increased number and size of lipid droplets in the brain induced by the expression of ND-23^KK102085 under the control of Scer\GAL4^repo.PU, are not suppressed by the co-expression of Pfk^UAS.cTa.

(Cabirol-Pol et al., 2018)

Co-expression of Pfk^Scer\UAS.cTa significantly improves the survival of flies expressing Scer\GAL4^elav-C155>ND-23^KK102085.

Co-expression of Zw^Scer\UAS.cLa significantly improves the survival of flies expressing Scer\GAL4^elav-C155>ND-23^KK102085.

(Besson et al., 2015)

Xenogenetic Interactions

Statement

Reference

The decreased lifespan, the decreased locomotor capacity and the ommatidium defects observed in adults expressing ND-23^KK102085 under the control of Scer\GAL4^elav.PU are partially or fully suppressed by the co-expression of Hsap\SLC2A3^UAS.cBa.

(Cabirol-Pol et al., 2018)

Co-expression of Hsap\SLC2A3^Scer\UAS.cBa significantly suppresses the life-expectancy defects resulting from the expression of Scer\GAL4^elav-C155>ND-23^KK102085.

(Besson et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v110797

P{KK102085}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ND-23^KK102085

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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