UAS regulatory sequences drive expression of an Aβ peptide (1-38). The Hsap\APP sequence in the construct is codon-optimized for expression in Drosophila melanogaster. A consensus Drosophila melanogaster translation start site is used. The signal sequence used in the construct is Tag:SS(rPENK).
Flies expressing Scer\GAL4nSyb.PS>Hsap\APP1-38.Scer\UAS.T:Rnor\SS-PENK display median lifespan similar to that of wild-type.