FB2025_01 , released February 20, 2025
Allele: Dmel\robo1ΔIg4.Tag:HA
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General Information
Symbol
Dmel\robo1ΔIg4.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0321742
Feature type
allele
Associated gene
Dmel\robo1
Associated Insertion(s)
Carried in Construct
P{robo1-HA.ΔIg4}
Key Links
Transgenic product class
inframe_deletion
(Reichert et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Reichert et al., 2016)
Mutagen
in vitro construct
(Reichert et al., 2016)
Progenitor genotype
Carried in construct
P{robo1-HA.ΔIg4}
Cytology
Description

2385bp of robo1 upstream flanking sequence drives expression of robo1 lacking the Ig4 domain (amino acid residues 345-440 are missing). 2192bp of robo1 downstream flanking sequence is also present. The protein produced contains the endogenous robo1 signal peptide and is tagged with 4 copies of Tag:HA directly upstream of the first Ig domain present (Ig1).

(Reichert et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
robo1
Encoded product / tool
robo1
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of robo1ΔIg4.T:Ivir\HA1 in the wild-type background has no discernible effect on the structure and morphology of the embryonic ventral nerve cord, not even when two copies of the transgene are present.

      (Reichert et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The midline collapse phenotype observed in the ventral nerve cord of embryos expressing comm10xScer\UAS.cRa under the control of Scer\GAL4elav.PLu is not affected by combination with robo1ΔIg4.T:Ivir\HA1.

      (Reichert et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      robo1ΔIg4.Tag:HA rescues robo11

      (Reichert et al., 2016)
      Comments

      Expression of robo1ΔIg4.T:Ivir\HA1 in robo11 mutant background fully rescues the defects in midline repulsion characteristic for robo11 mutants and restores the normal appearance of the axon scaffold in the embryonic ventral nerve cord.

      (Reichert et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      robo1ΔIg4.T:Ivir\HA1
      robo1ΔIg4.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)