FB2025_01 , released February 20, 2025
Allele: Dmel\kek634
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General Information
Symbol
Dmel\kek634
Species
D. melanogaster
Name
FlyBase ID
FBal0338015
Feature type
allele
Associated gene
Dmel\kek6
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Ulian-Benitez et al., 2017)
Mutagen
FLPase
(Ulian-Benitez et al., 2017)
Progenitor genotype
PBac{RB}e00907
(Ulian-Benitez et al., 2017)
PBac{WH}f05733
(Ulian-Benitez et al., 2017)
Cytology
Description

Recombination between the FRT sites present in the two progenitor insertions has resulted in a 41.9kb deletion that removes the entire kek6 open reading frame.

(Ulian-Benitez et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      kek634/Df(3R)ED6361 transheterozygous larvae are more frequently still and crawl at significantly lower average speeds (despite being able to achieve the same fastest speeds), as compared to controls. There are several defects observed in their larval NMJs compared to controls: targeting defects, including loss of projections and misrouting/mistargeting; NMJ anatomy defects, including significant decreases in the number of type Ib boutons, in the axonal length, in the number of NMJ branch points and in synaptic vesicle release and a significant increase in the number of active zones and a small increase in the number of ghost boutons.

      (Ulian-Benitez et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT Enhancer of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      spz537/Df(3L)Exel6092, kek634/Df(3R)ED6361 double transheterozygotes are viable and larvae exhibit an impaired locomotion phenotype (more frequent immobility and slower average speed) which is similar to that observed in spz537/Df(3L)Exel6092 transheterozygotes (although travelling shorter distances) and more severe than that observed in kek634/Df(3R)ED6361 transheterozygotes. These double mutants also show larval NMJ defects that are similar to those of spz537/Df(3L)Exel6092 and more severe than those of kek634/Df(3R)ED6361.

      The decrease in type I bouton number observed at the larval neuromuscular junction of kek634/Df(3R)ED6361 transheterozygotes is suppressed by the expression of Vap33Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4Toll-6-D42.

      The decrease in type I bouton number observed at the larval neuromuscular junction of spz537/Df(3L)Exel6092, kek634/Df(3R)ED6361 double transheterozygotes is suppressed by either the expression of kek6UAS.mRFP(Unk) under the control of Scer\GAL4Toll-6-D42 or by expression of Vap33Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4elav.PU, and is enhanced by the expression of Vap33Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4Toll-7. The decrease in axon length observed at the larval neuromuscular junction of these double transheterozygotes is also suppressed by the expression of either kek6UAS.mRFP(Unk) or CaMKIIT287D.Scer\UAS under the control of Scer\GAL4Toll-6-D42.

      (Ulian-Benitez et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      kek634/Df(3R)ED6361 is partially rescued by kek6UAS.mRFP(Unk)/Scer\GAL4Toll-6-D42

      (Ulian-Benitez et al., 2017)
      Comments

      The decreases in type Ib bouton number and axonal length, but not the small increase in the number of ghost boutons, observed at the larval NMJ of kek634/Df(3R)ED6361 transheterozygotes are partially rescued by the expression of kek6UAS.mRFP(Unk) under the control of Scer\GAL4Toll-6-D42.

      (Ulian-Benitez et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      kek634
      (Ulian-Benitez et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)