UASt regulatory sequences drive expression of the Hsap\ATP6AP1 open reading frame. The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein. The ORF was obtained from the human ORFeome v7.1 collection ( ORF_ID:3574 ) and corresponds to Ensembl transcript ENST00000369762.7. The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.