FlyBase Allele Report: Dmel\Rdh1[UAS.Tag:FLAG]

General Information

Symbol

Dmel\Rdh1^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0373213

Feature type

allele

Associated gene

Dmel\Rdh1

Associated Insertion(s)

Carried in Construct

P{UAS-Flag.Rdh1}

Key Links

Transgenic product class

cDNA

(Zhao et al., 2022)

wild_type

(Zhao et al., 2022)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhao et al., 2022)

wild_type

(Zhao et al., 2022)

Mutagen

in vitro construct

(Zhao et al., 2022)

Progenitor genotype

Carried in construct

P{UAS-Flag.Rdh1}

Cytology

Description

UASt regulates expression of a Rdh1 cDNA tagged with Tag:FLAG at the N-terminus.

(Zhao et al., 2022)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rdh1

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^ppl.PP

(Zhao et al., 2022)

lipid droplet | third instar larval stage, with Scer\GAL4^ppl.PP

(Zhao et al., 2022)

Detailed Description

Statement

Reference

The expression of Rdh1^UAS.Tag:FLAG under the control of Scer\GAL4^ppl.PP induces large lipid droplets in the fat body of third instar larvae fed on a high-fat diet.

The expression of Rdh1^UAS.Tag:FLAG under the control of Scer\GAL4^ppl.PP leads to fewer peripheral lipid droplets in the third instar larval fat body.

(Zhao et al., 2022)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Rdh1^UAS.Tag:FLAG, Scer\GAL4^ppl.PP has lipid droplet | third instar larval stage phenotype, suppressible by mdy^UAS.cHa, Scer\GAL4^ppl.PP

(Zhao et al., 2022)

Rdh1^UAS.Tag:FLAG, Scer\GAL4^ppl.PP has embryonic/larval fat body | third instar larval stage phenotype, suppressible by mdy^UAS.cHa, Scer\GAL4^ppl.PP

(Zhao et al., 2022)

Rdh1^UAS.Tag:FLAG, Scer\GAL4^ppl.PP has lipid droplet | third instar larval stage phenotype, suppressible by Lsd-2^GFP

(Zhao et al., 2022)

Rdh1^UAS.Tag:FLAG, Scer\GAL4^ppl.PP has embryonic/larval fat body | third instar larval stage phenotype, suppressible by Lsd-2^GFP

(Zhao et al., 2022)

Other

Statement

Reference

Rdh1^UAS.Tag:FLAG, Scer\GAL4^ppl.PP, mdy^UAS.cHa has embryonic/larval fat body | third instar larval stage phenotype

(Zhao et al., 2022)

Rdh1^UAS.Tag:FLAG, Scer\GAL4^ppl.PP, mdy^UAS.cHa has lipid droplet | third instar larval stage phenotype

(Zhao et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The double expression of mdy^UAS.cHa and Rdh1^UAS.Tag:FLAG under the control of Scer\GAL4^ppl.PP leads to enlarged lipid droplets in the third instar larval fat body; there are fewer peripheral lipid droplets.

(Zhao et al., 2022)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rdh1^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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