FlyBase Allele Report: Dmel\CG3740[CRISPR]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\CG3740^CRISPR

General Information

Symbol

Dmel\CG3740^CRISPR

Species

D. melanogaster

Name

FlyBase ID

FBal0399915

Feature type

allele

Associated gene

Associated Insertion(s)

TI{TI}CG3740^CRISPR

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

(Sreejith et al., 2024)

gene targeting by homologous recombination

(Sreejith et al., 2024)

Progenitor genotype

Associated Insertion(s)

TI{TI}CG3740^CRISPR

Cytology

Description

134bp deletion within the CG3740 coding sequence. Generated using a donor plasmid based on pHD-DsRed-attP. The Disc\RFP^{DsRed(T1).3xP3.cHa} marker has been excised, but a small fragment of the pHD-DsRed-attP-based donor plasmid remains at the site of the deletion.

(Sreejith et al., 2024)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | adult stage

(Sreejith et al., 2024)

decreased rate of adult locomotory behavior | progressive

(Sreejith et al., 2024)

increased mortality | nutrition conditional

(Sreejith et al., 2024)

(Sreejith et al., 2024)

Phenotype Manifest In

(Sreejith et al., 2024)

lipid droplet | adult stage

(Sreejith et al., 2024)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

CG3740^CRISPR, Nazo^KO has short lived phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^KO has abnormal starvation stress response | adult stage phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^KO has abnormal oxidative stress response | adult stage phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^KO has decreased rate of adult locomotory behavior | progressive phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^KO has increased mortality | nutrition conditional phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^KO has increased size | adult stage | nutrition conditional phenotype

(Sreejith et al., 2024)

Phenotype Manifest In

Suppressed by

Statement

Reference

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype, suppressible by Scer\GAL4^NP0001/bmm^JF01946

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype, suppressible by Scer\GAL4^NP0001/bmm^JF01946

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype, suppressible by Lsd-2^UAS.cGa/Scer\GAL4^NP0001

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype, suppressible by Lsd-2^UAS.cGa/Scer\GAL4^NP0001

(Sreejith et al., 2024)

NOT suppressed by

Statement

Reference

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype, non-suppressible by dob^HMC06191, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype, non-suppressible by dob^HMC06191, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype, non-suppressible by sturkopf^UAS.EGFP, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype, non-suppressible by sturkopf^UAS.EGFP, Scer\GAL4^Act5C.PI

(Sreejith et al., 2024)

Other

Statement

Reference

CG3740^CRISPR, Nazo^KO has adult midgut phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has adult midgut phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^RNAi.UAS.cSa, Scer\GAL4^Act5C.PI has lipid droplet | adult stage phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^KO has adult fat body phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^KO has lipid droplet | adult stage phenotype

(Sreejith et al., 2024)

CG3740^CRISPR, Nazo^KO has crop | adult stage | nutrition conditional phenotype

(Sreejith et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

CG3740^CRISPR

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections