TCGCATCCCGCTCATCCCGCCGCTCATCCGCAGGGTCCGCCGCCGCCGGGCGCCTATCGCCGTTACCCCTACTATCAACA CGCGGGTCCGCCGCCGCCGCACGAGCTCTACGAACAGCAACCGCCCCCGCCGCCCGTCTCATCCGGATCGGTGTCCGGGC CGGCGCAGCAGCAGCCGCCCACTAGCAGCCCAGCATCTGTTGGCGCAGCCGGAGCAGGAACAGCGGGAGCACCATCTGGC AGCCAGTTGGTACCGGCCCATCAGCAACAGCACCAGCAGCAGCACTTGGAGCACTATCCGGGACCGCCTAGCTACTACGC CGGATACAGTCCCGGCGGAGGAGCATCCGCCGGTCAGTGCTACACGCGTGGACTGCAAGGACCGTATATGGAATATCCAC CGCAGTGTCCCTGCCCAATGCCTCAATCGTGTCCAAAAAACGTCCATACTGGGCCTCATATTGGTAGCAACATCATCAGC AACATCGATCAGAGCAGCAGCTGCAGCAGCAGTATGTTGCTGAGTGCCACGAGCAACAGCAACATCGGCGGCGGCAGCAG CAGCAGCAGCAGCAGCAGCAGCAGCACCACCTTGCCTGCCTGCAGCAGCGCTACGACAACAACTACAGCGTCAGCGTTGA CTACCAGTAGAGGCCCAGTGAAAAATGTGACCCCCAACAACAGCAACAGTAGCGGCCACACCAGCACCAACAGTTGCAGG AGCAGCAACAACAGCAACAACAGCAGTAACAGCAGCTGTCAGACGATTGAGATGACCTCATGGGCAACAGCAGCAACCAA CACGACAAGCACGACGGCGACAACCGCCACAACAGGCACCCAGTGCCAGATGCTGGTGAAGACGGAGCTGAAGGGCGAGA ATCCTCAGGTTACCAAAATGCAGTACGAGGCGCACGTAATGCCGCCGCCCACTCCGCCGGAAAGCTATTGTGCCAGTGAC GAGAAGCTGCTCGATGACCAGGAGCAGGAGGCCGAGCTGGAGCTCCGCCTGCGTCTGGGATTGGGCCTGGAGAAGAGTCA CGGTGGGCAGCAGCACATCGAGGCGTACGATCTCACCTGTAGCACCCCGCCCCCAGTCACCGTGCCCCAGGCAGCAGCAG TGCCGCGCAAGGCGAGAATTGGCAAGAGCATGGCCAGGGAAATGGTCTATGCTGCCCAGCAGCAGCTGCTTCCGGAGAAG CAGTTGAAGCAGCCAAAGGAGGCGGCTGCGAAACTAGAAGCGGAACCAGAGATGCCTTTCGTACTTAAAGCGGAAATCAA AGCAGAGACCAAAATCAAAATCGAGCACGACGGCGAGGCGCAGGCCAGCACACATATCAGCCAACTGCCGGACCTGAAGC CCACCATCAAAACGGAGCCAGAGCAGGAACTGGAGCAAGAAGCGGCATCAGTACCGTCTGTGGCCAAAACGGAACCGCAA CCAGAAGTGGAGGTCGAGCGGGAACAACAGGAACCGCCGGTTGACGAGGAGCAACAGCCTACGCCAGGCGGTAGCAAGCA ACGCTTCAACCTGCTGGCCTTGTCCAGCGGCAACAAAAAGACGAAAACAAACAACAGCTACAAGAGCCTGATCAAGCAGG CGGAGCCCAAGAGTTACCTGTGCCCCGGTCGCAGGTTCATCCGTCGACACGGACGTGCTCCGGCCAAATATGTCAAACGG CGTCAGATGATTCTGACGCAGCGGCAGCAGCAGCGAATGCGTCTGCAACGCCGCGAGCAACAGCAGCAACGTCGCCAGCA ACGCGAAGCGGCTGCAGAAGTAGTTGCAGGAGCTGTTGTCACCCCATCTCTGGCAGGAAACCCCATCGAGCAGGCAGGAT CAGAAGTTTCCATCGTTAAAGATCAAGCACCCAACAGCTCGGCCACGCCAAGTGCATCGCCGAAAAGAGCACGCCCCCGG AAACAGGAGATTGCTGCTCTGCATCTGCTCGAAGGCTTGGATACGACGCTGAGTCGTGGTTACTTCAGTGACCCGGAACT GAATCACAGTAGTCGCAAGCAGGCGCCGACAGCTGTGGGTGGGCTGTATGCCGCGTCCTCGCCGCTAACTACGGCGACCG ATCACAGGGGCAAACAGACGACTGCCGAGAAGCGCTCCAAGTCGGAGACCAAGAAGCCACCGTCGGGCGAAAGTTCCGAG TCAGGTCTGCCGTGCAAGAAGCCGCGCAAAGAGCCTGCTGGCAAGGTGAAGGGTAAGGGCAAGAAGACAACGGCGGGAGC AGCAGCGACAACAATTGCGTCCACAGCTGAAACTCAGACGGAGGCCGTAAATGCCATCACTGCATCCAACCAGGAGACGA ACAACAACGAGTATCAGACGGCAGATCTGCAGGCTCAAAAGTTCCGTGAGTGTCCGGCCGTCACTGATGCAAAAGTAGCG GGAACAGCAACATCCACATCCACAGCCTCACAGCAGCAGAACCAGCAACATTCCGACGAGCAACACCAGTTCCTGGTGCC CAGTCAGCCAGCGACGGCCAGCGCCACCGCTGCCCTAGCAGCCACTCCTCCGCCCAGCCGTCAAGGTCAGGCGACACACA CAAAGCGGACCAGATCGCGCTGCAAGTTCGGGAATCGAAAGCGCCAAAGACACAGACCTGGCGGCGTAAGCTACGAGCTG GATGTGACCCAGCCGCCGGCCACCAAGGCCAATGTGGTGCCCAAGTGGAACAATGGATGGATGTGGGCCGGCAAGGCGTT CCAGGGTGCTGTCTTTCTCAATAGCGATGATCCGCTGGTGCTGCGCACCTGCTACCCGGCCATGCGGCACGTGGAGGGCG ACATTATCCGCATCAGGGACTGTGTGCTGCTCAAGGCCAACGAGGACAATGAGCTGCCATACGTGGCCAAAGTGGCGCAC CTCTGGCAGAATCCCGAGGACGGCGAGATGATGATGTCGCTGCTGTGGTACTACCGACCCGAGCATACCGATCAGGGACG GCAGCGCAACGATTGTCCGGACGAGGTCTATGCCTCCCGCCATCGCGATCACAACTCGGTGGCATGCGTCGAGGACAAGT GCTACGTGCTCACCTTCAGCGAGTACTGCAGATATCGTCGCCGCCTACGTGCCGCCGAGGAGGATGTGGAAGACGTAAGC ATTGTGCCGCGTCGGCCAAGCATTGCCACCGCTCCCGGGTTTCCAGTGCGAACGGTGCCGGAACACACCAATCCGGAGCT GGTGATGTTCTGCCGCCGAGCCTATGAGTTCCGGACGCGACGTCTGCTCAAGCTGCCCCATAAGAACGGGCTCGTGTGCA GCTCCAGCTAGCGCAAGGCATGAGCCATTGCGATCGAGCTCCCGTCAACCCAGGCAATGGATGAACTGCGGAGTGTAGGA ACAATAGCTTAAATTCAGATTGCAGTGGGAATTGGATATTGATATGCGGAGTGGCGATGGAGAAAGCCCTGGCAGAGGGG ATGGACAAGGAACTACTCATAGAGAGAAAGTTACATTGTTTTGATCAGTCTACAGTATTGTTAAAGATATTTTTTGTCTT TGTTGTTAAGAGATATCTGCAAGGATGGGCCCCCGGGCTGTGCATCTGCATTAGTTGTTCCTAACCAAATATCGATTGCT CTTGTTGCGCCCGTCGTTCGATGACGCGGACACGGACACGTACACTACCTCTGTCGGGGCAGGATAAGGGACACACAGAT ACACCCGGGACCCCAAGTATACCCAGGACACACACACACACACACACACACACAAACATACACCAGACCAAAATGTAGGC GAAGGCTTTTCACTTAGACGTGAGAATTTCGAGAGATGCGAGGAGAAGAGGGAAAATGTTGGGAGACAACTGCTAGGCGA AAGCATTAAATACTTTACATTGTATTACATAATTTATTTATTCTGACTACACACCCCACCCCCTGCCAACCCTCACACAC ATGCGGATATTTTTGTTATTAAGTTGTATGTATTTATTATCATTAATATCGAGTAAGTCAGCATTCTGTGTACAGTTTTC CGCTCGCTTTTGCTCTAATCGCTCAAGGAATTGGTTTAACCACCTACCGAAAACCACATCCATTAGCTAAGACCCTTTCG ATGGCCAAAAAGTTATTTGGTGTGCCACAGAAATCTCTGCAAATCGACTTGATGCGATTTTAATTGTCTTTTCCTCAAAA CTAAATCCATTTGCTCTGTAGAGATCACTGCGGCTCTCCGAATAAGAACAAGCTTTCAACTTTTTTTTTTTTTTTAAACT AAAAAGTGTTCGGATAGTCGTTGAAATAGTTTTTTTATTTTGGTATAACCGATTTTTAAATCGCCAAATCGAGTCTACCG ACTTTAAATTGTAATGATTATCGTGCATGCCTAATAAAGAACTTGGAATATTCCGATAAATATTTTAGGAATAGCTGAAT TAACCATTATTTTATATACTTGACCAACGGTTTCCACTTTTTCCATTTACACTCTAAATGAGATATCCTTCTCTGTCGTT TAATTTTCGATAGATTCAGATGCATGAAAGACCAGGCTTAACCCATTGATGGCCAGGCAAAAAACTTTGCGCACAGACAA AACCAATCTAACTATAATTTCTGACATATTTGAGATTTCTGGCTCCAGAGGCGGAAAACTGGATGGCTTGACATCGATCC TTGCTAGGCGCTGTTGGTATATATTTTTATTTGTTGTTAGTTTTTGTAAGCGATTGAACACACATTTTGTACTCTTGATT TGACATTTATCTTTTGTGTTCGCCTTAGTTTGGAAATTTGATTTCAGAGACATGATTTTCTTTCGGTTAACTATATTATT CAGCTGCAAACAGTTAACGAGAATCTTTAGGGAAAATAACAACAGAAACAACAACAAGTCACATTATTATATTATATATA CATAAATATATCTACGTAGTTCTATATAAACAAATATCCTTATCCATAAAACATTTTTTTGTAATCTTAATTTAATTTAG CAGTAGCGTTTTTAGTTGCTAAGTTGATATTGCAAATAGTTTGTTTTATACCAAATACACAAAAAAAAAAAAAAAAAAAA AAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
