FB2025_01 , released February 20, 2025
Clone: Dmel\RE10237
Open Close
General Information
Symbol
Dmel\RE10237
Species
D. melanogaster
Name
RE10237
FlyBase ID
FBcl0193025
Feature type
Computed gene(s)
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    RE_cDNA
    • RE cap-trapped cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr).
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,085
      NCBI
      Full Length sequence dataSequence Downloader
      AGTGGTCAACTCCGCTGCTGCAGATCCACAGCCAAAAAATGCTGCACATAGTATCGGTTGTTATTATAGCCCTGAGTCTG
      GGCTGTGCCGTTGGCCAGGATTTGCGGCGTGTGGACGACACCGAGTTGATTCAACTCCTGACCGGCAGCAGCAATGTGGT
      TGTCCTATTCAGTGAGTATTGGTAGGAAAGTCAGCCAGAGAAATCCAGCCTACGTGTCCACTGTGCATATGGAGGATGGT
      ACACCGGTTACTGGCTAACGAGCACACTTAATTGCAGACAAAAACAATTGCCAGCGCTGCGTGGAATATGAGAACATGGT
      GACCAAGATACGTGCCCAGCTGGAGGAGACCTTGTCGGCCATTGTGGTCCAGTCGGTGGACAGCAACCTGGTATCCATCT
      ATGACCCCAGCAAGGAGCCCGCTTTGGTGTTCTTCAGGCGAGGAATACCCATTCTATACCATGGCGAGATCAACGATGAC
      GAAATCCTAGACTTCTTCAATGACAACTTAGAGCCGGCGGTCAAAGAGCTTTCCGACGACAACTTCGAGCACCTGACTCA
      AGCCTCGTCGGGTGCCACTACCGGGGACTGGTTTATCTTCTTCTCTTCGGCCGAATGTACCGTCTGTCAGCGACTCTATG
      CCGTTTGGGAGTCTGTGGGCGGAAAGCTAAAGCGAAAGCTGAATATTGCCCGCATGAACAGTTTGGAGTCTGGAATCTCA
      ACTGCTACGCGACTGGGAGTATTGGAAGCACCGGCTTTTATTTTCTTGAGGCAAGGAAAGATGTACAAATATTCAGCCAA
      GCAATACAGTCCCGAAGCCTTTGTCCAGTTTGCTGAGAAGGGATATACCCAGAGCCATCCTCAACCAGTGCCCGCAATCC
      CAAGTGCCGTAAACGAATTTTTGGGCCCACTGCAAAGTTATTTTGCAGCTGAAAACATAACATCGCTGGCGACTTTGAGT
      ATTTTCGCCTTAGTTGCTCTCTTATTCGTTGGCAAAAAGGTTGCCAAAATATTCAGTTCAGAGCCTGCGAAAACAAAAAA
      TAAGTCGAAATAAAACTAATATAAAGACCAAAAAAAAAAAAAAAA
      
      Library Information (1)
      Library: RE_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hr embryos.

      Sample preparation

      For the RE cDNA library, embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was polyA+ selected twice (RNA made by L. Hong). cDNA was synthesized by priming with the oligo(dT) primer adapter (5'-GAGAGAGAGAGGATCCAATACTGGAGAGTTTTTTTTTTTTTTTTVN-3'). The first strand was synthesized in presence of trehalose, which increases the full-length cDNA synthesis (Carninci, P. et al. 1998. Proc. Natl. Acad. Sci. U.S.A. 95(2): 520-524; Carninci, P. et al. 1999. Methods Enzymol. 303: 19-44). Full-length cDNA was selected with the biotinylated cap-trapper (Carninci, P. et al. 1996. Genomics 37(3): 327-336). A linker was then ligated to the single-strand cDNA following the published protocol (Shibata, Y., et al. 2001. Biotechniques 30(6): 1250-1254). Subsequently, the cDNA was normalized by using RoT=1.0 as published (Carninci, P., et al. 2000. Genome Res. 10(10): 1617-1630). Second strand cDNA was primed with the (5'-AGAGAGAGAGCTCGAGCTCTAATAAGGTGACACTATAGAACCA-3') primer. After restriction digestion of the hemimethylated cDNA with BamHI and XhoI, the cDNA was cloned in the lambda FLC-I vector. Subsequently, the library was bulk-excised into pFLC-I plasmid as described (Carninci, P., et al. 2001. Genomics 77(1-2): 79-90). cDNAs were transformed into DH5-alpha TonA strain.

      Comment
      Reported As
      Symbol Synonyms
      RE_cDNA
      Riken embryo library
      References (3)
      Research paper (2)
      Hoskins et al., 2011, Genome Res. 21(2): 182--192
      Genome-wide analysis of promoter architecture in Drosophila melanogaster. [FBrf0213090]
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      Supplementary material (1)
      Hoskins et al., 2011, Genome Res. 21(2):
      Supplemental Material. [FBrf0213251]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)