FB2025_01 , released February 20, 2025
Clone: Dmel\Dmel_dig_005169_0593_2554
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General Information
Symbol
Dmel\Dmel_dig_005169_0593_2554
Species
D. melanogaster
Name
Dmel_dig_005169_0593_2554
FlyBase ID
FBcl0377267
Feature type
cDNA_clone
Computed gene(s)
Dmel\ND-MWFE
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    TTT_DIG_cDNA
    • RNA-Seq of D. melanogaster, iso-1 strain, adult virgin female, 3' ends of polyadenylated transcripts.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | virgin female
      organism

      Comment: 3-7d old

      (Torres et al., 2008)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      93
      NCBI
      EV579056
      3' sequence dataSequence Downloader
      CATGGACGAGCGTTTCAGCCGAGTTATGTACCAGCGCGATTTCCGACTGACCGACAATCCCTACAAGATGAACGGTCTGG
ATGCCATACCGGA
      Library Information (1)
      Library: TTT_DIG_cDNA
      Description

      Sequence of 3' ends of cDNAs prepared from total RNA from adult females.

      (Torres et al., 2008)
      Sample preparation
      Protocol

      Total RNA was isolated and double-stranded cDNA was prepared using an oligo-dT primer for first-strand synthesis. Preparations were digested separately with Mbo I, Nla III and Tai I, and 3' fragments recovered. The 3' fragments produced by each restriction enzyme were pooled before sequencing.

      (Torres et al., 2008)
      Comment
      Reported As
      Symbol Synonyms
      TTT_DIG
      (Torres et al., 2008)
      TTT_DIG_cDNA
      References (1)
      Research paper (1)
      Torres et al., 2008, Genome Res. 18(1): 172--177
      Gene expression profiling by massively parallel sequencing. [FBrf0202153]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)