Source was yeast cell line; bait produced from DNA containing 3\'UTR of bcd; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Three-hybrid system: yeast RevM10-GAL4-BD/GAL4-AD
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Bound RNA identified by microarray, defined as having over five-fold enrichment in immunoprecipitates compared to negative controls, with FDR of 5%.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Bound RNA identified by microarray, defined as having over two-fold enrichment in immunoprecipitates compared to negative controls, with FDR of 5%.
H606A,K608A, coordinates relative to stau-PA
K628E,K629E, coordinates relative to stau-PA
R742A,R744A, coordinates relative to stau-PA
K764E,K765A,K768A, coordinates relative to stau-PA
S581A, coordinates relative to stau-PA
Q718A, coordinates relative to stau-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey RNA commercially synthesized in vitro.
