Co-expression of hh or smoCT reduces interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
The ci construct used contains Serine-to-Alanine mutations in three PKA phosphorylation sites to prevent proteolytic processing.
This interaction is inhibited by the co-expression of activated fu constructs.
Bait expressed in transiently transfected cells.
Prey expressed in transiently transfected cells.
