FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Polr2A, Nelf-E
FlyBase ID
FBig0000095925
Interaction Network
Interactions Browser links
Polr2A network
Nelf-E network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0216979-4.coIP.WB
Reference
Cernilogar et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Polr2A 

RNA Pol II

bait
Nelf-E 

NELF-E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line nuclear extracts.

(Cernilogar et al., 2011)

RNase treatment did not disrupt observed interaction.

(Cernilogar et al., 2011)
FBrf0240455-1.coIP.WB
Reference
Nazer et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Nelf-E 

NELF-E

bait

HA tag

Polr2A 

Pol II

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Nazer et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)