FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Zasp52, Actn
FlyBase ID
FBig0000097011
Interaction Network
Interactions Browser links
Zasp52 network
Actn network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0202682-1.coIP.WB
Reference
Jani and Schock, 2007
Description
physical association
Assay
Collection
Source/Stage
organism / first instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zasp52 

Zasp52

bait
Actn 

α-actinin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was first instar larval extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Jani and Schock, 2007)
FBrf0221051-1.coIP.WB
Reference
Katzemich et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zasp52 

Zasp52

bait

GFP tag

Actn 

α-actinin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST-tagged Zasp52 also interacts in vitro with rabbit α-actinin.

(Katzemich et al., 2013)

Source was adult thorax of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Katzemich et al., 2013)
FBrf0227292-027.TFIN
Reference
Rhee et al., 2014
Description
physical association
Assay
Collection
TF_Interaction_Network-2
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Actn 

FBgn0000667

unspecified role
Actn 

FBgn0000667

unspecified role
Actn 

FBgn0000667

unspecified role
Zasp52 

FBgn0083919

unspecified role
Zasp52 

FBgn0083919

unspecified role
Zasp52 

FBgn0083919

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

(Rhee et al., 2014)

HGScore = 21.620647

(Rhee et al., 2014)
FBrf0233858-1.coIP.WB
Reference
Liao et al., 2016
Description
physical association
Assay
Collection
Source/Stage
adult thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zasp52 

Zasp52

bait

FLAG tag

Zasp52 
Actn 

α-actinin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Zasp52 
PDZ and first LIM domain
sufficient binding region
aa 1-449

coordinates relative to Zasp52-PK

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult thorax of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Liao et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)