FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
fra, Abl
FlyBase ID
FBig0000098886
Interaction Network
Interactions Browser links
fra network
Abl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0183931-1.PD.WB
Reference
Forsthoefel et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fra 

Fra

bait

GST tag

fra 
Abl 

Abl

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fra 
cytoplasmic domain
sufficient binding region
aa 1098-1375
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

(Forsthoefel et al., 2005)

fra binds to Abl and trio, but not ena in this assay.

(Forsthoefel et al., 2005)
FBrf0183931-5.coIP.WB
Reference
Forsthoefel et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

bait

Myc tag

fra 

Fra

prey

HA tag

fra 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fra 
cytoplasmic domain
necessary binding region
aa 1123-1375
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Forsthoefel et al., 2005)

fra binds to Abl and trio, but not ena in this assay.

(Forsthoefel et al., 2005)
FBrf0183931-7.PD.WB
Reference
Forsthoefel et al., 2005
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fra 

Fra

bait

GST tag

fra 
Abl 

Abl

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
fra 
cytoplasmic domain
sufficient binding region
aa 1098-1375
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult head extract.

(Forsthoefel et al., 2005)
FBrf0210365-1.coIP.WB
Reference
Dorsten et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
fra 

Fra

bait

HA tag

Abl 

DAbl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Dorsten et al., 2010)
FBrf0221894-1.coIP.WB
Reference
O'Donnell and Bashaw, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
fra 

Fra

bait

Myc tag

Abl 

Abl

prey

GFP tag

Abl 
Abl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abl 
N-terminal region
sufficient binding region
aa 1-644
Abl 
SH2 motif
mutation disrupting interaction
aa 297

R297K

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(O'Donnell and Bashaw, 2013)

Mutations to Abl kinase or SH3 domains do not disrupt binding to fra.

(O'Donnell and Bashaw, 2013)

Mutation of tyrosine residues in the fra cytoplasmic domain does not disrupt the interaction with Abl.

(O'Donnell and Bashaw, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)