FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
l(3)mbt, Lint-1
FlyBase ID
FBig0000101630
Interaction Network
Interactions Browser links
l(3)mbt network
Lint-1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0218194-1.AP.MS
Reference
Meier et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
l(3)mbt 

dL(3)mbt

bait

FLAG tag

Lint-1 

dLint-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Meier et al., 2012)
FBrf0218194-12.coIP.WB
Reference
Meier et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
Lint-1 

dLint-1

bait

FLAG tag

l(3)mbt 

dL(3)mbt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Meier et al., 2012)
FBrf0218194-16.coIP.WB
Reference
Meier et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lint-1 

dLint-1

bait
l(3)mbt 

dL(3)mbt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Meier et al., 2012)
FBrf0218194-2.PD.A
Reference
Meier et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(3)mbt 

dL(3)mbt

bait

FLAG tag

Lint-1 

dLint-1

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and insect cell system; prey produced and labeled by in vitro translation.

(Meier et al., 2012)
FBrf0218194-22.CH.WB
Reference
Meier et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc
Participants
Corresponds to
Reported as
Role
Note
Lint-1 

dLint-1

unspecified role
Lint-1 

dLint-1

unspecified role
Lint-1 

dLint-1

unspecified role
l(3)mbt 

dL(3)mbt

unspecified role
l(3)mbt 

dL(3)mbt

unspecified role
l(3)mbt 

dL(3)mbt

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Meier et al., 2012)

Lint-1 fractionates in two peaks suggesting two Lint-1 containing complexes. Lint-1 co-fractionates with l(3)mbt in one peak.

(Meier et al., 2012)
FBrf0218194-25.coIP.WB
Reference
Meier et al., 2012
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lint-1 

dLint-1

bait
l(3)mbt 

dL(3)mbt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Meier et al., 2012)
FBrf0218194-3.coIP.WB
Reference
Meier et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(3)mbt 

dL(3)mbt

bait

FLAG tag

Lint-1 

dLint-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from infected recombinant baculovirus; prey produced from infected recombinant baculovirus.

(Meier et al., 2012)
FBrf0218194-4.AP.MS
Reference
Meier et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Lint-1 

dLint-1

bait

FLAG tag

l(3)mbt 

dL(3)mbt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Meier et al., 2012)
FBrf0218194-5.coIP.WB
Reference
Meier et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Lint-1 

dLint-1

bait

FLAG tag

l(3)mbt 

dL(3)mbt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Meier et al., 2012)
FBrf0227292-396.TFIN
Reference
Rhee et al., 2014
Description
physical association
Assay
Collection
TF_Interaction_Network-2
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
l(3)mbt 

FBgn0002441

unspecified role
l(3)mbt 

FBgn0002441

unspecified role
l(3)mbt 

FBgn0002441

unspecified role
Lint-1 

FBgn0030274

unspecified role
Lint-1 

FBgn0030274

unspecified role
Lint-1 

FBgn0030274

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

(Rhee et al., 2014)

HGScore = 28.02951

(Rhee et al., 2014)
FBrf0254671-1.coIP.MS
Reference
Yamamoto-Matsuda et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
OSC
Participants
Corresponds to
Reported as
Role
Note
l(3)mbt 

l(3)mbt

bait
Lint-1 

Lint-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Identification of l(3)mbt co-repressors was discerned by additional mass spectrometry analysis after RNAi of l(3)mbt resulting in a 4-fold reduction along with Gene Ontology categorization of DNA-binding and chromatin regulation.

(Yamamoto-Matsuda et al., 2022)

Source was of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Yamamoto-Matsuda et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)