To visualize the interaction, both proteins were tagged with a complementary fragment of the Venus fluorophore. Proximity of the two proteins resulted in reconstitution of the fluorescent protein.

Interaction in embryo; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

Gel free pull down assay using biotin labeled DNA consensus sites. Complex was pulled down with streptavidin and Scr-Dll heterodimer identified with an anti-FLAG antibody.

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.