FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cac, Rbp
FlyBase ID
FBig0000105517
Interaction Network
Interactions Browser links
cac network
Rbp network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0216992-1.Y2H
Reference
Liu et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cac 

CAC

bait

fused to GAL4 DNA-binding domain

cac 
cac 
Rbp 

RBP

prey

fused to GAL4 activation domain

Rbp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cac 
C-terminal region
sufficient binding region
aa 1420-1768

"Δ80aa" construct, coordinates relative to cac-PA

cac 
C-terminal region
necessary binding region
aa 1689-1768

"Δ160aa" construct, coordinates relative to cac-PA

Rbp 
SH3-III
sufficient binding region
aa 1383-1552

coordinates relative to Rbp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Liu et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Liu et al., 2011)
FBrf0229297-2.Y2H
Reference
Siebert et al., 2015
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbp 

RBP

bait

fused to LexA DNA-binding domain

Rbp 
cac 

Cacophony

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbp 
SH3-II and SH3-III domains
sufficient binding region
aa 1302-1510

coordinates relative to Rbp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult head cDNA expression library).

(Siebert et al., 2015)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Siebert et al., 2015)
FBrf0229297-5.ITC
Reference
Siebert et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbp 

RBP

bait
Rbp 
Rbp 
cac 

Cac

prey
cac 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbp 
SH3-II domain
sufficient binding region
aa 1301-1383

Kd = 20.2 uM, coordinates relative to Rbp-PF

Rbp 
SH3-III domain
sufficient binding region
aa 1442-1510

Kd = 17.3 uM, coordinates relative to Rbp-PF

cac 
PxxP motif
sufficient binding region
IGRRLPPTPSKPSTL
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey synthesized in vitro..

(Siebert et al., 2015)

The Rbp-cac is much weaker than the Rbp-Aplip1 interaction.

(Siebert et al., 2015)
FBrf0229297-8.XC
Reference
Siebert et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbp 

RBP

neutral component
Rbp 

RBP

neutral component
Rbp 
Rbp 

RBP

neutral component
Rbp 
cac 

Cac

neutral component
cac 
cac 

Cac

neutral component
cac 

Cac

neutral component
cac 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbp 
SH3-III domain
sufficient binding region
aa 1442-1510

coordinates relative to Rbp-PF

cac 
PxxP motif
sufficient binding region
IGRRLPPTPSKPSTL
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey synthesized in vitro..

(Siebert et al., 2015)
FBrf0245633-5.Y2H
Reference
Petzoldt et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbp 

RIM-BP

bait

fused to lexA DNA-binding domain

Rbp 
Rbp 
cac 

Cac

prey

fused to GAL4 activation domain

cac 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbp 
SH3-II domain
sufficient binding region
aa 1040-1382

coordinates relative to Rbp-PF

Rbp 
SH3-III domain
sufficient binding region
aa 1441-1507

coordinates relative to Rbp-PF

cac 
C-terminal domain
sufficient binding region
aa 1500-1848
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/GAL4-AD. Positive control.

(Petzoldt et al., 2020)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Petzoldt et al., 2020)
FBrf0245633-6.Y2H
Reference
Petzoldt et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cac 

Cac

bait

fused to lexA DNA-binding domain

cac 
Rbp 

RIM-BP

prey

fused to GAL4 activation domain

Rbp 
Rbp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbp 
SH3-II domain
sufficient binding region
aa 1040-1382

coordinates relative to Rbp-PF

Rbp 
SH3-III domain
sufficient binding region
aa 1441-1507

coordinates relative to Rbp-PF

cac 
C-terminal domain
sufficient binding region
aa 1500-1848
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/GAL4-AD. Positive control.

(Petzoldt et al., 2020)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Petzoldt et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)