FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000107011
Interaction Network
Interactions Browser links
Tob network
Pop2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0224133-43.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pop2 

Pop2

bait

V5 tag

Tob 

Tob

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The orb2 interacting domain of Tob is dispensable for the Tob-Pop2 interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0235788-496.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tob 

Tob-RC

bait

fused to GAL4 DNA-binding domain

Pop2 

Pop2-RB

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0235788-496.Y2Hpool
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tob 

Tob-RC

bait

fused to GAL4 DNA-binding domain

Pop2 

Pop2-RB

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)