FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
TBPH, Nipped-B
FlyBase ID
FBig0000107307
Interaction Network
Interactions Browser links
TBPH network
Nipped-B network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0233521-11.coIP.WB
Reference
Swain et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
ML-DmBG3-c2
Participants
Corresponds to
Reported as
Role
Note
Nipped-B 

Nipped-B

bait
TBPH 

TBPH

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Nipped-B coimmunoprecipitates with both TBPH and lark, but no TBPH-lark interaction was observed.

(Swain et al., 2016)

Source was cell extract of ML-DmBG3-c2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Swain et al., 2016)
FBrf0233521-9.PD.WB
Reference
Swain et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
ML-DmBG3-c2
Participants
Corresponds to
Reported as
Role
Note
TBPH 

TBPH

bait

His tag

TBPH 
Nipped-B 

Nipped-B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
TBPH 
RRM1
sufficient binding region
aa 1-190

coordinates relative to TBPH-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from ML-DmBG3-c2 cell extract.

(Swain et al., 2016)

The interaction is not affected by RNase treatment and is thus RNA-independent.

(Swain et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)