Source was cell extract of COS cell line; bait produced from transfected construct; prey produced from transfected construct.

When co-transfected, tkv and put form a heteromeric complex with [125]I-labeled BMP2. Alone, tkv binds BMP2 only weakly, and put does not bind BMP2 at all.

Cells were transfected with tkv and put then incubated with [125]I-labeled human BMP2 and crosslinked by disuccinimidyl suberate. Proteins binding to BMP2 are radiolabeled in SDS-PAGE.

Source was cell extract of COS cell line; bait produced from transfected construct; prey produced from transfected construct.

When co-transfected, tkv and put form a heteromeric complex with [125]I-labeled BMP2. Alone, tkv binds BMP2 only weakly, and put does not bind BMP2 at all.

Cells were transfected with tkv and put then incubated with [125]I-labeled human BMP2 and crosslinked by disuccinimidyl suberate. Proteins binding to BMP2 are radiolabeled in SDS-PAGE.