FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-bft, Nach
FlyBase ID
FBig0000108799
Interaction Network
Interactions Browser links
mir-bft network
Nach network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0234481-1.miR-mRNA.PCR
Reference
Kim et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult midgut / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-bft 

miR-263a

bait
Nach 

Nach

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutation of bft increases Nach mRNA levels (41-fold).

(Kim et al., 2017)

Source was adult midgut of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kim et al., 2017)
FBrf0234481-2.miR-mRNA.WB
Reference
Kim et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult midgut / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-bft 

miR-263a

bait
Nach 

Nach

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutation of bft increases Nach protein levels.

(Kim et al., 2017)

Source was adult midgut of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kim et al., 2017)
FBrf0234481-3.miR-mRNA.LUC
Reference
Kim et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mir-bft 

miR-263a

bait
Nach 

Nach

prey
Nach 
Nach 
Nach 
Nach 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nach 
3-prime UTR
sufficient binding region
Nach 
mir-263a target site 1
necessary binding region
CGAAUGCAAAAAAAUAUUGCCAUA
Nach 
mir-263a target site 2
necessary binding region
ACCCGCGAGUUGCAAAUAUCUAUCCUACUAUCGCAUAUUGCCAUA
Nach 
mir-263a target site 3
necessary binding region
GUCCGUGGGGUAUAAAAAGGGGAAAUUGCUAUG
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Luciferase reporter assay.

(Kim et al., 2017)

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Kim et al., 2017)
External Crossreferences and Linkouts ( 0 )
References (1)