FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
egl, bcd
FlyBase ID
FBig0000108917
Interaction Network
Interactions Browser links
egl network
bcd network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0234579-11.coIP.SEQ
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

bcd 

bcd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
FBrf0234579-2.coIP.PCR
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

bcd 

bcd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
FBrf0243139-8.coIP.PCR
Reference
Goldman et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

RFP tag

egl 
egl 
bcd 

bcd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
interaction domain residues 965,969
mutation decreasing interaction
aa 965,969

S965K,S969R, coordinates relative to egl-PB

egl 
N-terminal domain residue 35
mutation decreasing interaction
aa 35

C35Y, coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries from transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Goldman et al., 2019)
FBrf0251875-7.coIP.PCR
Reference
Goldman et al., 2021
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

FLAG tag

egl 
bcd 

bcd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
RNA binding domain residues 676-726
mutation decreasing interaction
nt 676-726

truncated RNA binding domain;coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Goldman et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)