FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pgc, Cdk9
FlyBase ID
FBig0000116186
Interaction Network
Interactions Browser links
pgc network
Cdk9 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0202665-2.coIP.WB
Reference
Hanyu-Nakamura et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pgc 

Pgc

bait

FLAG tag

Cdk9 

Cdk9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hanyu-Nakamura et al., 2008)
FBrf0202665-6.coIP.WB
Reference
Hanyu-Nakamura et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pgc 

Pgc

bait

FLAG tag

Cdk9 

Cdk9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Hanyu-Nakamura et al., 2008)
FBrf0202665-8.PD.WB
Reference
Hanyu-Nakamura et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pgc 

Pgc

bait

MBP tag

Cdk9 

Cdk9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Hanyu-Nakamura et al., 2008)
FBrf0202665-9.PD.WB
Reference
Hanyu-Nakamura et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pgc 

Pgc

bait

MBP tag

Cdk9 

Cdk9

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

(Hanyu-Nakamura et al., 2008)
External Crossreferences and Linkouts ( 0 )
References (1)