FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pgc, CycT
FlyBase ID
FBig0000116326
Interaction Network
Interactions Browser links
pgc network
CycT network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0202665-1.coIP.WB
Reference
Hanyu-Nakamura et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pgc 

Pgc

bait

FLAG tag

CycT 

CycT

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hanyu-Nakamura et al., 2008)
FBrf0202665-3.coIP.WB
Reference
Hanyu-Nakamura et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
CycT 

CycT

bait

FLAG tag

pgc 

Pgc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hanyu-Nakamura et al., 2008)
FBrf0202665-5.coIP.WB
Reference
Hanyu-Nakamura et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pgc 

Pgc

bait

FLAG tag

CycT 

CycT

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Hanyu-Nakamura et al., 2008)
FBrf0202665-7.PD.WB
Reference
Hanyu-Nakamura et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pgc 

Pgc

bait

MBP tag

CycT 

CycT

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct in S2 cell extract.

(Hanyu-Nakamura et al., 2008)
External Crossreferences and Linkouts ( 0 )
References (1)